Sequence-specific 1H, 15N and 13C resonance assignments of the 23.7-kDa homodimeric toxin CcdB from Vibrio fischeri
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CcdB is the toxic component of a bacterial toxin–antitoxin system. It inhibits DNA gyrase (a type II topoisomerase), and its toxicity can be neutralized by binding of its antitoxin CcdA. Here we report the sequential backbone and sidechain 1H, 15N and 13C resonance assignments of CcdBVfi from the marine bacterium Vibrio fischeri. The BMRB accession number is 16135.
KeywordsCcdB Toxin–antitoxin systems Chromosomal ccd operon Homodimeric protein
Funding by the Austrian Science Foundation (FWF) under project number 19902 to K.Z. is gratefully acknowledged. L. B. is a postdoctoral fellow of the Research Foundation-Flanders (FWO) N.D.J. acknowledges receipt of PhD grants from IWT and the Onderzoeksraad of the VUB. Further financial support was provided by FWO-Vlaanderen and VIB.
- Buts L, Lah J, Dao-Thi MH et al (2005) Toxin-antitoxin modules as bacterial metabolic stress managers. Trends Biochem Sci 30:672–679Google Scholar
- De Jonge N, Buts L, Vangelooven J et al (2007) Purification and crystallization of Vibrio fischeri CcdB and its complexes with fragments of gyrase and CcdA. Acta Crystallogr 63:356–360Google Scholar