Skip to main content
SpringerLink
Log in

NMR assignments of the periplasmic loop P2 of the MalF subunit of the maltose ATP binding cassette transporter

  • Article
  • Published:
Biomolecular NMR Assignments Aims and scope Submit manuscript

Abstract

We have assigned the 1H, 15N, 13C backbone resonances of the second periplasmic loop P2 of the MalF subunit of the maltose ATP binding cassette transporter of Escherichia coli/Salmonella which is important for the recognition of the maltose binding protein MalE.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Abbreviations

ABC:

ATP binding cassette

MalF-P2:

Second periplasmic loop of MalF

MalF, MalG:

Transmembrane subunits of the maltose transporter

MalE:

Maltose binding protein

MalK:

Intracellular nucleotide binding subunit

NBD:

Nucleotide binding domain

References

  • Boos W, Lucht JM (1996) Periplasmic binding protein-dependant ABC transporters. In: Neidhardt FC, Curtiss R, Ingraham JL, Lin ECC, Low KB, Magasanik B (eds) E. coli and salmonella typhimurium: cellular and molecular biology. American Society for Microbiology Press, Washington, DC, pp 1175–1209

    Google Scholar 

  • Cornilescu G, Delaglio F, Bax A (1999) Protein backbone angle restraints from searching a database for chemical shift and sequence homology. J Biomol NMR 13:289–302. doi:10.1023/A:1008392405740

    Article  Google Scholar 

  • Daus ML, Landmesser H, Schlosser A, Müller P, Herrmann A, Schneider E (2006) ATP induces conformational changes of periplasmic loop regions of the maltose ATP-binding cassette transporter. J Biol Chem 281:3856–3865. doi:10.1074/jbc.M511953200

    Article  Google Scholar 

  • Froshauer S, Green GN, Boyd D, McGovern K, Beckwith J (1988) Genetic analysis of the membrane insertion and topology of malf, a cytoplasmic membrane protein of Escherichia coli. J Mol Biol 200:501–511. doi:10.1016/0022-2836(88)90539-6

    Article  Google Scholar 

  • Higgins CF (1992) ABC TRANSPORTERS—from microorganisms to man. Annu Rev Cell Biol 8:67–113. doi:10.1146/annurev.cb.08.110192.000435

    Article  Google Scholar 

  • Oldham ML, Khare D, Quiocho FA, Davidson AL, Chen J (2007) Crystal structure of a catalytic intermediate of the maltose transporter. Nature 450:515–522. doi:10.1038/nature06264

    Article  ADS  Google Scholar 

  • Sattler M, Schleucher J, Griesinger C (1999) Heteronuclear multidimensional NMR experiments for the structure determination of proteins in solution employing pulsed field gradients. Prog NMR Spect 34:93–158. doi:10.1016/S0079-6565(98)00025-9

    Article  Google Scholar 

  • Shilton BH, Shuman HA, Mowbray SL (1996) Crystal structures and solution conformations of a dominantnegative mutant of E. coli maltose-binding protein. J Mol Biol 264:364–376. doi:10.1006/jmbi.1996.0646

    Article  Google Scholar 

  • Vranken WF, Boucher W, Stevens TJ, Pajon RFA, Llinas M, Ulrich EL, Markley JL, Ionides J, Laue ED (2005) The CCPN data model for NMR spectroscopy: development of a software pipeline. Proteins 59:687–696. doi:10.1002/prot.20449

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by the Leibniz-Gemeinschaft and the DFG (grants Re1435, Schn274/9–3, SFB 449, SFB 740)

Author information

Authors and Affiliations

  1. Leibniz-Institut für Molekulare Pharmakologie (FMP), Robert-Rössle-Str. 10, D-13125, Berlin, Germany

    Tomas Jacso, Peter Schmieder & Bernd Reif

  2. Institut für Biologie, AG Bakterienphysiologie, Humboldt-Universität zu Berlin, Chausseestr. 117, D-10115, Berlin, Germany

    Mathias Grote & Erwin Schneider

  3. Charité Universitätsmedizin, D-10115, Berlin, Germany

    Bernd Reif

Authors
  1. Tomas Jacso
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mathias Grote
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Peter Schmieder
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Erwin Schneider
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bernd Reif
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bernd Reif.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jacso, T., Grote, M., Schmieder, P. et al. NMR assignments of the periplasmic loop P2 of the MalF subunit of the maltose ATP binding cassette transporter. Biomol NMR Assign 3, 21–23 (2009). https://doi.org/10.1007/s12104-008-9131-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12104-008-9131-7

Keywords

Search

Navigation