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Biomolecular NMR Assignments

, Volume 3, Issue 1, pp 1–3 | Cite as

NMR assignment of prespore specific antigen—a cell surface adhesion glycoprotein from Dictyostelium discoideum

  • James D. Swarbrick
  • Liza Cubeddu
  • Graham E. Ball
  • Paul M. G. Curmi
  • Andrew A. Gooley
  • Keith L. Williams
  • Bridget C. Mabbutt
Article

Abstract

Presopore-specific antigen (PsA) is a cell surface glycoprotein of the cellular slime mould Dictyostelium discoidum implicated in cell adhesion. The 15N, 13C and 1H chemical shift assignments of PsA were determined from multidimensional, multinuclear NMR experiments. Resonance assignments have been made for both the N-terminal globular domain and its attached O-glycosylated PTVT linker motif.

Keywords

Glycopeptide linker GPI-anchor Ponticulin N-acetyl glucosamine 

Notes

Acknowledgements

We thank Brett Collins for assisting in the preparation of rPsA and Murray Coles for helpful discussions. The work was supported by a project grant from the Australian Research Council.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • James D. Swarbrick
    • 1
    • 2
  • Liza Cubeddu
    • 3
  • Graham E. Ball
    • 4
  • Paul M. G. Curmi
    • 5
  • Andrew A. Gooley
    • 6
  • Keith L. Williams
    • 6
  • Bridget C. Mabbutt
    • 1
  1. 1.Department of Chemistry & Biomolecular SciencesMacquarie UniversitySydneyAustralia
  2. 2.Medicinal Chemistry and Drug Action, Monash Institute of Pharmaceutical SciencesMonash UniversityMelbourneAustralia
  3. 3.School of Molecular and Microbial BiosciencesUniversity of SydneySydneyAustralia
  4. 4.School of ChemistryUniversity of New South WalesSydneyAustralia
  5. 5.School of PhysicsUniversity of New South WalesSydneyAustralia
  6. 6.SGE Analytical Science Pty LtdRingwoodAustralia

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