NMR assignment of prespore specific antigen—a cell surface adhesion glycoprotein from Dictyostelium discoideum
Presopore-specific antigen (PsA) is a cell surface glycoprotein of the cellular slime mould Dictyostelium discoidum implicated in cell adhesion. The 15N, 13C and 1H chemical shift assignments of PsA were determined from multidimensional, multinuclear NMR experiments. Resonance assignments have been made for both the N-terminal globular domain and its attached O-glycosylated PTVT linker motif.
KeywordsGlycopeptide linker GPI-anchor Ponticulin N-acetyl glucosamine
We thank Brett Collins for assisting in the preparation of rPsA and Murray Coles for helpful discussions. The work was supported by a project grant from the Australian Research Council.
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