NMR assignments of HIV-2 TAR RNA
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We report nearly complete assignment for all 1H, 13C, 31P, and 15N resonances in the 30-nucleotide stem-loop HIV-2 TAR RNA located at the 5′ end of all viral mRNAs.
KeywordsHIV-2 TAR RNA synthesis RNA resonance assignment
The authors thank Lincoln Scott (Cassia LLC, San Diego) and Edit Sperling (TSRI, La Jolla) for preparing nucleotide triphosphates for transcription reactions, Claudia Schwiegk (MPI, Goettingen) for the syntesis of the TAR RNA used in 13C detected experiments and Christophe Fares (UHN, Toronto) for the development of the 13C detected experiments. This work was supported by a grant from the National Institutes of Health (GM-66669, to M.H. and J.R.W), by the Deutsche Forschungsgemeinschaft (SFB416 to T.C.) and by the MPG.