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Biomolecular NMR Assignments

, Volume 2, Issue 2, pp 155–157 | Cite as

NMR assignments of the sylvatic dengue 1 virus envelope protein domain III

  • David E. Volk
  • Kurtis M. Anderson
  • Sai H. A. Gandham
  • Fiona J. May
  • Li Li
  • David W. C. Beasley
  • Alan D. T. Barrett
  • David G. GorensteinEmail author
Article

Abstract

Nearly complete backbone and side chain resonance assignments have been obtained for the third domain, residues M289–K400, of the envelope protein from the sylvatic strain (P72–1244) of the dengue 1 virus, containing mutations N336S and E370K, using double- and triple-resonance spectroscopy.

Keywords

Dengue Flavivirus Nuclear magnetic resonance assignments Envelope protein 

Notes

Acknowledgments

This work was funded by the Pediatric Dengue Vaccine Initiative, the Centers for Disease Control (U90CCU618754), the National Institute for Allergic and Infectious Diseases (U01 AI054827), the Welch Foundation (H1296), and the State of Texas Advanced Technology Program (004952-0038-2003). We thank Sean Moran for assistance with the Rice University spectrometer whose carbon-enhanced HCN cold probe was obtained through the Strategic Partnership for Research in Nanotechnology Grant AFRL (AFOSR) FA9550-04-1-0328.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • David E. Volk
    • 1
    • 2
  • Kurtis M. Anderson
    • 1
  • Sai H. A. Gandham
    • 1
  • Fiona J. May
    • 3
  • Li Li
    • 3
  • David W. C. Beasley
    • 4
    • 5
  • Alan D. T. Barrett
    • 3
    • 4
  • David G. Gorenstein
    • 1
    • 2
    Email author
  1. 1.Department of Biochemistry and Molecular BiologyThe University of Texas Medical BranchGalvestonUSA
  2. 2.Sealy Center for Structural Biology and Molecular BiophysicsThe University of Texas Medical BranchGalvestonUSA
  3. 3.Department of PathologyThe University of Texas Medical BranchGalvestonUSA
  4. 4.Sealy Center for Vaccine DevelopmentThe University of Texas Medical BranchGalvestonUSA
  5. 5.Department of Microbiology and ImmunologyThe University of Texas Medical BranchGalvestonUSA

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