NMR assignment of the nonstructural protein nsp3(1066–1181) from SARS-CoV
Sequence-specific NMR assignments of the globular core comprising the residues 1066–1181 within the non-structural protein nsp3e from the SARS coronavirus have been obtained using triple-resonance NMR experiments with the uniformly [13C, 15N]-labeled protein. The backbone and side chain assignments are nearly complete, providing the basis for the ongoing NMR structure determination. A preliminary identification of regular secondary structures has been derived from the 13C chemical shifts.
KeywordsSevere acute respiratory syndrome SARS coronavirus Nonstructural protein NMR structure determination
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