Biomolecular NMR Assignments

, Volume 2, Issue 2, pp 135–138 | Cite as

NMR assignment of the nonstructural protein nsp3(1066–1181) from SARS-CoV

  • Pedro Serrano
  • Margaret A. Johnson
  • Amarnath Chatterjee
  • Bill Pedrini
  • Kurt Wüthrich
Article

Abstract

Sequence-specific NMR assignments of the globular core comprising the residues 1066–1181 within the non-structural protein nsp3e from the SARS coronavirus have been obtained using triple-resonance NMR experiments with the uniformly [13C, 15N]-labeled protein. The backbone and side chain assignments are nearly complete, providing the basis for the ongoing NMR structure determination. A preliminary identification of regular secondary structures has been derived from the 13C chemical shifts.

Keywords

Severe acute respiratory syndrome SARS coronavirus Nonstructural protein NMR structure determination 

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Pedro Serrano
    • 1
  • Margaret A. Johnson
    • 1
  • Amarnath Chatterjee
    • 1
  • Bill Pedrini
    • 1
  • Kurt Wüthrich
    • 2
  1. 1.Department of Molecular BiologyThe Scripps Research InstituteLa JollaUSA
  2. 2.Department of Molecular Biology and Skaggs Institute for Chemical BiologyThe Scripps Research InstituteLa JollaUSA

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