Advertisement

Biomolecular NMR Assignments

, Volume 1, Issue 2, pp 225–227 | Cite as

NMR assignment of Cdc42(T35A), an active Switch I mutant of Cdc42

  • Paul D. AdamsEmail author
  • Robert E. Oswald
Article

Abstract

Cdc42(T35A) is an active construct of Cdc42, a Ras GTPase involved in signal transduction, containing a single-point mutation in an important effector-binding region. We determined the backbone and side chain resonance assignments of 13C,15N-labelled Cdc42(T35A) from E. coli.

Keywords

Cdc42(T35A) Ras GTPase Signal transduction 

Notes

Acknowledgements

The authors thank Drs. Robert McFeeters, Adriene Loh, Ahmed Ahmed, TKS Kumar, and Roger Koeppe II for helpful discussions. We also thank Mrs. Shatara Porchia-White for technical assistance.

References

  1. Barbacid M (1987) ras Genes. Ann Rev Biochem 56:779–827CrossRefGoogle Scholar
  2. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:277–293CrossRefGoogle Scholar
  3. Feltham JL, Dotsch V, Raza S, Manor D, Cerione RA, Sutcliffe MJ, Wagner G, Oswald RE (1997) Definition of the switch surface in the solution structure of Cdc42Hs. Biochemistry 36:8755–8766CrossRefGoogle Scholar
  4. Guo W, Sutcliffe MJ, Cerione RA, Oswald RE (1998) Identification of the binding surface on Cdc42Hs for p21-activated kinase. Biochemistry 37:14030–14037CrossRefGoogle Scholar
  5. Hart M, Eva A, Evans T, Aaronson S, Cerione R (1991) Catalysis of guanine nucleotide exchange on the Cdc42Hs Protein by the dbl Oncogene Product. Nature 354:311–314CrossRefADSGoogle Scholar
  6. Hart M, Shinjo K, Hall A, Evans T, Cerione RA (1991) Identification of the human platelet GTPase activating protein for the CdC42Hs protein. J Biol Chem 266:20840–20848Google Scholar
  7. Leonard D, Hart MJ, Platko JV, Eva A, Henzel W, Evans T, Cerione RA (1992) The identification and characterization of a GDP-dissociation inhibitor (GDI) for the CDC42Hs protein. J Biol Chem 267:22860–22868Google Scholar
  8. Loh AP, Guo W, Nicholson LK, Oswald RE (1999) Backbone dynamics of inactive, active, and effector-bound Cdc42Hs from measurements of (15)N relaxation parameters at multiple field strengths. Biochemistry 38:12547–12557CrossRefGoogle Scholar
  9. Mccormick F (1989) ras GTPase activating protein: signal transmitter and signal terminator. Cell 56:5–8CrossRefGoogle Scholar
  10. Wittinghofer A, Pai E (1991) The structure of Ras protein: a model for a universal molecular Switch. Trends Biomed Sci 16:382–387CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Department of Chemistry and BiochemistryUniversity of Arkansas-FayettevilleFayettevilleUSA
  2. 2.Department of Molecular MedicineCornell University College of Veterinary MedicineIthacaUSA

Personalised recommendations