NMR assignment of Cdc42(T35A), an active Switch I mutant of Cdc42
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Cdc42(T35A) is an active construct of Cdc42, a Ras GTPase involved in signal transduction, containing a single-point mutation in an important effector-binding region. We determined the backbone and side chain resonance assignments of 13C,15N-labelled Cdc42(T35A) from E. coli.
KeywordsCdc42(T35A) Ras GTPase Signal transduction
The authors thank Drs. Robert McFeeters, Adriene Loh, Ahmed Ahmed, TKS Kumar, and Roger Koeppe II for helpful discussions. We also thank Mrs. Shatara Porchia-White for technical assistance.
- Hart M, Shinjo K, Hall A, Evans T, Cerione RA (1991) Identification of the human platelet GTPase activating protein for the CdC42Hs protein. J Biol Chem 266:20840–20848Google Scholar
- Leonard D, Hart MJ, Platko JV, Eva A, Henzel W, Evans T, Cerione RA (1992) The identification and characterization of a GDP-dissociation inhibitor (GDI) for the CDC42Hs protein. J Biol Chem 267:22860–22868Google Scholar