Biomolecular NMR Assignments

, Volume 1, Issue 2, pp 225–227 | Cite as

NMR assignment of Cdc42(T35A), an active Switch I mutant of Cdc42

  • Paul D. AdamsEmail author
  • Robert E. Oswald


Cdc42(T35A) is an active construct of Cdc42, a Ras GTPase involved in signal transduction, containing a single-point mutation in an important effector-binding region. We determined the backbone and side chain resonance assignments of 13C,15N-labelled Cdc42(T35A) from E. coli.


Cdc42(T35A) Ras GTPase Signal transduction 



The authors thank Drs. Robert McFeeters, Adriene Loh, Ahmed Ahmed, TKS Kumar, and Roger Koeppe II for helpful discussions. We also thank Mrs. Shatara Porchia-White for technical assistance.


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Department of Chemistry and BiochemistryUniversity of Arkansas-FayettevilleFayettevilleUSA
  2. 2.Department of Molecular MedicineCornell University College of Veterinary MedicineIthacaUSA

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