Biomolecular NMR Assignments

, Volume 1, Issue 2, pp 171–173 | Cite as

NMR assignment of human chemerin, a novel chemoattractant

  • Samantha J. Allen
  • Brian A. Zabel
  • John Kirkpatrick
  • Eugene C. Butcher
  • Daniel Nietlispach
  • Tracy M. Handel
Article

Abstract

Chemerin is a potent chemoattractant for cells expressing the GPCR CMKLR1, and is thought to play important roles in cell migration and recruitment to sites of tissue damage and inflammation. Here we report the NMR assignments of the 15.6 kDa active form of uniformly 15N, 13C labeled chemerin.

Keywords

Chemerin Chemotaxis Inflammation CMKLR1 

Notes

Acknowledgements

We thank David King (UC-Berkeley, CA) for mass spectrometry analysis, Peter Domaille (Diversa, San Diego, CA) for NMR data collection and Damon Hamel (UC-San Diego, CA) for help with data processing. This work was funded by Cancer Research Institute and Universitywide AIDS Research Program (TF06-SD-501) fellowships to S.J.A, and a National Institutes of Health grant RO1-AI37113 to T.M.H. B.A.Z. and E.C.B are funded by National Institutes of Health grants AI-59635, AI-47822, HL-67674 and GM-37734.

References

  1. Kulig P, Zabel BA, Dubin G, Allen SJ, Ohyama T, Potempa J, Handel TM, Butcher EC, Cichy J (2007) Staphylococcus aureus-derived staphopain B, a potent cysteine protease activator of plasma chemerin. J Immunol 178:3713–3720Google Scholar
  2. Martensson UE, Fenyo EM, Olde B, Owman C (2006) Characterization of the human chemerin receptor-ChemR23/CMKLR1-as co-receptor for human and simian immunodeficiency virus infection, and identification of virus-binding receptor domains. Virology 355:6–17CrossRefGoogle Scholar
  3. Meder W, Wendland M, Busmann A, Kutzleb C, Spodsberg N, John H, Richter R, Schleuder D., Meyer M, Forssmann WG (2003) Characterization of human circulating TIG2 as a ligand for the orphan receptor ChemR23. FEBS Lett 555:495–499CrossRefGoogle Scholar
  4. Samson M, Edinger AL, Stordeur P, Rucker J, Verhasselt V, Sharron M, Govaerts C, Mollereau C, Vassart G, Doms RC, Parmentier M (1998) ChemR23, a putative chemoattractant receptor, is expressed in monocyte-derived dendritic cells and macrophages and is a coreceptor for SIV and some primary HIV-1 strains. Eur J Immunol 28:1689–1700CrossRefGoogle Scholar
  5. Vranken WF, Boucher W, Stevens TJ, Fogh RH, Pajon A, Llinas M, Ulrich EL, Markley JL, Ionides J, Laue ED (2005) The CCPN data model for NMR spectroscopy: development of a software pipeline. Proteins 59:687–696CrossRefGoogle Scholar
  6. Wittamer V, Franssen J-D, Vulcano M, Mirjolet J-F, Le Poul E, Migeotte I, Brezillon S, Tyldesley R, Blanpain C, Detheux M, Mantovani A, Sozzani S, Vassart G, Parmentier M, Communi D (2003) Specific recruitment of antigen-presenting cells by chemerin, a novel processed ligand from human inflammatory fluids. J Exp Med 198:977–985CrossRefGoogle Scholar
  7. Wittamer V, Gregoire F, Robberecht P, Vassart G, Communi D, Parmentier M (2004) The C-terminal nonapeptide of mature chemerin activates the chemerin receptor with nanomolar potency. J Biol Chem 279:9956–9962CrossRefGoogle Scholar
  8. Zabel BA, Allen SJ, Kulig P, Cichy J, Handel TM, Butcher EC (2005a) Chemerin activation by serine proteases of the coagulation, fibrinolytic and inflammatory cascades. J Biol Chem 280:34661–34666CrossRefGoogle Scholar
  9. Zabel BA, Silverio AM, Butcher EC (2005b) Chemokine-like receptor 1 expression and chemerin-directed chemotaxis distinguish plasmacytoid from myeloid dendritic cells in human blood. J Immunol 174:244–251Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Samantha J. Allen
    • 1
  • Brian A. Zabel
    • 2
    • 3
  • John Kirkpatrick
    • 4
  • Eugene C. Butcher
    • 2
    • 3
  • Daniel Nietlispach
    • 4
  • Tracy M. Handel
    • 1
  1. 1.Skaggs School of Pharmacy and Pharmaceutical SciencesUniversity of California, San DiegoLa JollaUSA
  2. 2.Laboratory of Immunology and Vascular Biology, Department of PathologyStanford University School of MedicineStanfordUSA
  3. 3.Center for Molecular Biology and MedicineVeterans Affairs Palo Alto Health Care SystemPalo AltoUSA
  4. 4.Department of BiochemistryUniversity of CambridgeCambridgeUK

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