The Indian Journal of Pediatrics

, Volume 86, Issue 5, pp 433–438 | Cite as

Respiratory Pathogens in Infants Diagnosed with Acute Lower Respiratory Tract Infection in a Tertiary Care Hospital of Western India Using Multiplex Real Time PCR

  • Anuja A. SonawaneEmail author
  • Jayanthi Shastri
  • Sandeep B. Bavdekar
Original Article



To determine the frequency of respiratory pathogens in infants diagnosed with acute lower respiratory tract infections.


A prospective cross-sectional observational study was conducted in infants hospitalized with a diagnosis of acute lower respiratory tract infection (ALRTI), in a tertiary care hospital in a metropolitan city of Western India. Nasopharyngeal swabs were analyzed by multiplex real time polymerase chain reaction, for 18 viruses and 3 bacteria (H. influenzae type b, C. pneumoniae and M. pneumoniae). The entire data was entered in Microsoft excel sheet and frequencies were determined.


One hundred eligible infants were enrolled. Pathogens were detected in 82 samples, which included Respiratory syncytial viruses (RSV) A / B (35.4%), Human rhinovirus (25.6%), Adenovirus (22%), Human Parainfluenza viruses (11%), Human bocavirus (9.8), Human metapneumovirus A / B (8.5%), Influenza A (H1N1) pdm 09 (6.1%), Parechovirus (3.7%), Human coronaviruses (3.66%), Haemophilus influenzae type b (6.1%), Chlamydia pneumoniae (2.4%) and Mycoplasma pneumoniae (2.4%). Influenza A (other than H1N1), Influenza B, Human Coronavirus 229E and Enterovirus were not detected. The rate of coinfection was 34% and rhinovirus was the most common of the multiple pathogens.


Spectrum of viral etiologies of ALRTI is highlighted. Etiological diagnosis of ALRTI would enable specific antiviral therapy, restrict antibiotic use and help in knowing burden of disease.


Multiplex real time PCR Acute lower respiratory tract infection Infants Respiratory viruses RSV Co-infection 



The authors’ thank Puritan Medical Products, USA, for providing them flocked swabs with universal transport media (UT – 317).

Authors' Contributions

AAS: Assistance in conceptualization of the research project and developing protocol, conduct of research, data collection, interpretation of results, intellectual inputs in drafting the manuscript and approval of the final draft; JS: Conceptualized the research idea, supervised the conduct of the research, intellectual inputs in drafting the manuscript and approval of the final draft; SSB: Assistance in conceptualization of the research project and developing protocol, interpretation of results, intellectual inputs for improving the research paper and approval of the final draft.

Compliance with Ethical Standards

Conflict of Interest


Source of Funding

Intramural research funds.


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Copyright information

© Dr. K C Chaudhuri Foundation 2019

Authors and Affiliations

  1. 1.Department of MicrobiologyT.N. Medical College & B.Y.L. Nair HospitalMumbaiIndia
  2. 2.Department of PediatricsT.N. Medical College & B.Y.L. Nair HospitalMumbaiIndia

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