Red Blood Cell Alloimmunization in Multitransfused Pediatric Population in a Tertiary Care Hospital
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To estimate the prevalence and specificity pattern of red blood cell (RBC) alloimmunization among pediatric multitransfused patients, and to identify the factors associated with alloimmunization.
This was a descriptive cross-sectional study conducted among mutitransfused pediatric patients over a period of two years. The relevant clinical details of patients were collected, and RBC antibody screening was done. Samples with positive antibody screen were subjected to antibody identification. Patient factors were analysed to find any significant relation to the development of RBC alloimmunization.
Alloantibodies were obtained in 4 (6.35%) of the total 63 patients, and autoantibody in 1 (1.59%). The specificities of alloantibodies identified were all against Rh antigens-one each of anti E, anti c, anti Cw and anti D + anti C. A significant association was seen between development of alloimmunization and first transfusion at more than 2 y of age.
RBC alloimmunization against Rhesus (Rh) antigens is a significant problem for multitransfused children in our population. Extended RBC phenotyping at least for antigens of the Rh system and provision of antigen matched RBCs may be an option for such children, where ongoing transfusion requirement is anticipated.
KeywordsRBC alloimmunization Alloantibodies Pediatric population Multitransfused
AP was the principal investigator. SF helped in data collection, testing and analysis of results. SPS contributed to the analysis and interpretation of data. UKC reviewed the study and suggested modifications. LK reviewed the final study and gave inputs. Dr. AP Poornima and Dr. Shaiji PS will act as guarantors for this article.
Compliance with Ethical Standards
Written informed consent was obtained from the guardians of the children in the study and Institutional Ethical Committee clearance was also obtained.
Conflict of Interest
Source of Funding
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