The Indian Journal of Pediatrics

, Volume 80, Issue 5, pp 371–374 | Cite as

Bacteriological Profile and Antibiogram of Neonatal Septicemia

  • Narayan GyawaliEmail author
  • Raj Kumari Sanjana
Original Article



To determine the incidence of neonatal septicemia, identify the bacterial isolates and study their antimicrobial susceptibility pattern in neonates visiting out-patient door (OPD) and admitted to the pediatric ward and neonatal intensive care unit (NICU) of the College of Medical Sciences, Teaching Hospital, Chitwan, Nepal.


Blood culture of all neonates who were suspected for septicemia was performed. Isolate identification and antimicrobial susceptibility testing was done by standard microbiological method.


Out of the total 7,868 blood culture samples submitted to the microbiology laboratory, 1,572 (19.97 %) samples were from suspected cases of neonatal septicemia. The blood culture was positive for neonatal sepsis in 238 (15.13 %) samples. The incidence of gram positive and gram negative organisms were 44.1 % and 55.9 % respectively. Staphylococcus aureus remained the predominant isolate followed by Klebsiella spp. Most of the gram positive isolates exhibited higher resistance to penicillin and cephalosporin. Susceptibility to commonly used aminoglycosides and quinolones was found. Similarly, more than two third isolates of gram negative Enterobacteriaceae showed resistance to ampicillin. Third generation cephalosporin and aminoglycosides were found to be more satisfactory among gram negative organisms as compared to gram positive.


The high incidence of neonatal septicemia in the present setting reflects high magnitude of problem in central part of Nepal. Organisms isolated in the study exhibited higher resistance towards commonly used antimicrobials. High resistances to recommended drugs awares health care workers to seek for alternative antimicrobial agents. The periodic survey of etiological agents and their antibiotic susceptibility patterns is indeed necessary for the timely alarm of such type of problems.


Neonatal septicemia Bacteriological profile Antimicrobial resistance 


Conflict of Interest


Role of Funding Source



  1. 1.
    Agrawal R, Sarkar N, Deorary AK, Paul VK. Sepsis in newborn. Indian J Pediatr. 2001;68:1143–7.CrossRefGoogle Scholar
  2. 2.
    Ang OY, Ezike E, Asmar BI. Antibacterial resistance. Indian J Pediatr. 2004;71:229–39.PubMedCrossRefGoogle Scholar
  3. 3.
    Joshi SJ, Ghole VS, Niphadkar KB. Neonatal gram negative bacteremia. Indian J Pediatr. 2000;67:27–32.PubMedCrossRefGoogle Scholar
  4. 4.
    Colle JG, Marr W. Cultivation of bacteria. In: Mackie, Mc Cartney, eds. Practical Medical Microbiology. 13th ed; Vol. 2, Edinburg: Churchill Livingstone; 1989. pp. 141–60.Google Scholar
  5. 5.
    Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Test. 9th ed. Approved Standard. Wayne, PA: Clinical and Laboratory Standard Institute; 2006. (CLSI document no. M2-A9).Google Scholar
  6. 6.
    Agnihotri N, Kaistha N, Gupta V. Antimicrobial susceptibility of isolates from neonatal septicemia. Jpn J Infect Dis. 2004;57:273–5.PubMedGoogle Scholar
  7. 7.
    Arora U, Devi P. Bacterial profile of blood stream infections and antibiotic resistance pattern of isolates. J K Science. 2007;9:186–90.Google Scholar
  8. 8.
    Shrestha P, Das BK, Bhatta NK, et al. Clinical and bacteriological profiles of blood culture positive sepsis in newborns. J Nepal Paediatr. 2007;27:64–7.Google Scholar
  9. 9.
    Karki BM, Parija SC. Analysis of blood culture isolates from hospitalized neonates in Nepal. Southeast Asian J Trop Med Publ health. 1999;30:546–8.Google Scholar
  10. 10.
    Jain NK, Jain VM, Maheshwari S. Clinical profile of neonatal sepsis. Kathmandu University Med J. 2003;1:117–20.Google Scholar
  11. 11.
    Karthikegan G, Premkumar K. Neonatal sepsis: Staphylococcus aureus as the predominant pathogen. Indian J Pediatr. 2001;68:715–7.CrossRefGoogle Scholar
  12. 12.
    Ullmann U. Infection agents in hospitalized patients. Zentralbl Bakteriol Mikrobiol Hyg B. 1986;183:103–13.PubMedGoogle Scholar
  13. 13.
    Tsering DC, Chanchal L, Pal R, Kar S. Bacteriological profile of septicemia and the risk factors in neonates and infants in Sikkim. J Glob Infect Dis. 2011;3:42–5.PubMedCrossRefGoogle Scholar
  14. 14.
    Kayange N, Kamugisha E, Mwizamholya DL, Jeremiah S, Mshana SE. Predictors of positive blood culture and deaths among neonates with suspected neonatal sepsis in a tertiary hospital, Mwanza-Tanzania. BMC Pediatr. 2010; 4; 10:39.Google Scholar
  15. 15.
    World Health Organization. Acute respiratory infections in children: case management in hospitals in developing countries. Geneva: World Health Organization; 1990.Google Scholar
  16. 16.
    Rahman S, Hameed A, Roghani MT, Ullah Z. Multidrug resistant neonatal sepsis in Peshawar, Pakistan. Arch Dis Child Fetal Neonatal Ed. 2002;87:F52–4.PubMedCrossRefGoogle Scholar
  17. 17.
    Movahedian AH, Moniri R, Mosayebi Z. Bacterial culture of neonatal sepsis. Iranian J Publ Health. 2006;35:84–9.Google Scholar

Copyright information

© Dr. K C Chaudhuri Foundation 2012

Authors and Affiliations

  1. 1.Department of MicrobiologyNepal Medical College, AttarkhelKathmanduNepal
  2. 2.Department of MicrobiologyCollege of Medical Sciences, Teaching HospitalChitwanNepal

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