Differential expression of S1P receptor subtypes in human bladder transitional cell carcinoma
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Sphingosine 1 phosphate (S1P), S1P receptors (S1PRs) and their signaling pathways play an important role in the fate of cancer cells. The expression pattern of S1PR subtypes (S1PR1–S1PR5) may alter in cancer development stages, depending on the origin and the pathologic features of tumors. The present study aimed to examine the relationship between plasma S1P levels and the expression of S1PR subtypes in bladder tumors.
These changes were evaluated in terms of the pathologic grades and stages of human bladder cancer samples. For this, tumor biopsies from 41 new bladder cancer patients as well as 26 normal-looking bladder tissues were collected and processed for immunohistochemistry (IHC) and quantitative real-time RT-PCR of S1PR subtypes. Plasma S1P level was measured using liquid chromatography–tandem mass spectrometry (LC–MS/MS).
The results show that tissue S1PR1, S1PR2 and S1PR3 are over-expressed in all tumors regardless of their pathological grade (~ 3, ~ 6 and ~ 104 folds, respectively). These results were corroborated by IHC data showing accumulation of S1PR subtypes 1 and 2 in the tissues. Plasma S1P in the plasma samples from patients was in the range of control samples (Controls; 256 ± 47; patients, 270 ± 41).
Overexpression of S1PR1, S1PR2 and S1PR3 in bladder tumor biopsies which were corroborated with the pathological grades and stages may suggest that S1PR profile in tumor biopsies is a promising marker in the diagnosis of bladder carcinoma.
KeywordsSphingosine 1 phosphate S1P S1P receptor Bladder cancer Urothelial tumor Tumor grade
This research is part of the PhD thesis of Alireza Palangi, Department of Clinical Biochemistry, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran and has been partially financially supported by project number 5892 from the Urology and Nephrology Research Center, Shahid Labbafinejad Medical center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Compliance with ethical standards
Conflict of interest
The authors declare that there are no conflicts of interest concerning this study.
This study has been approved by the ethical committee of Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Informed consent was obtained from all participants included in this study.
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