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Clinical and Translational Oncology

, Volume 19, Issue 12, pp 1507–1517 | Cite as

Overexpression of dishevelled 2 is involved in tumor metastasis and is associated with poor prognosis in hepatocellular carcinoma

  • C. Zhang
  • C. Li
  • X. Chen
  • Y. Zhou
  • B. Yin
  • R. Ni
  • Y. Zhang
  • J. Liu
Research Article

Abstract

Purpose

Although hepatocellular carcinoma (HCC) is one of the most common malignant tumors, its molecular mechanism is still unknown. Dishevelled 2 (Dvl2) is one of the downstream targets of non-canonical Wnt signaling, which has been demonstrated to be of great importance in the progression of cancers. Nevertheless, the expression mechanisms and physiological significance of Dvl2 in HCC remain unclear.

Methods

Western blotting and immunohistochemistry were used to measure Dvl2 protein expression in HCC and adjacent normal tissues of 101 patients. Wound healing and transwell assays were used to determine cell migration and invasion.

Results

Dvl2 expression was upregulated in HCC tissues compared to the adjacent normal tissues. Moreover, its expression level was significantly correlated with histological grade (P = 0.042), metastasis (P = 0.005) and vein invasion (P = 0.009) in patients with HCC. Wound healing and transwell assays showed that knockdown of Dvl2 reduced cell migration and invasion in HepG2 cells. Finally, we confirmed that Dvl2 could regulate the migration and invasion of HCC cells by interacting with P62 in non-canonical Wnt signaling.

Conclusions

Our data showed that Dvl2 was overexpressed in HCC tissues and was also correlated with poor prognosis, suggesting that Dvl2 is a novel therapeutic target for HCC.

Keywords

Dishevelled 2 Hepatocellular carcinoma Prognosis Metastasis 

Notes

Acknowledgements

This work was supported by the National Natural Scientific Foundation of China (No. 81401985) and Six talent peaks project in Jiangsu Province (WSN-057).

Compliance with ethical standards

Conflict of interest

The authors have no potential conflict of interest to declare.

Informed consent

All patients selected for our study were fully informed about our experiment protocols, which were approved by Ethics Committee of the ethics committee at our hospital, and provided written informed consent from each patient.

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Copyright information

© Federación de Sociedades Españolas de Oncología (FESEO) 2017

Authors and Affiliations

  1. 1.Grade 14, Clinical Medicine, Medical CollegeNantong UniversityNantongPeople’s Republic of China
  2. 2.Department of Hepatic OncologyAffiliated Cancer Hospital of Nantong UniversityNantongPeople’s Republic of China
  3. 3.Department of GastroenterologyAffiliated Hospital of Nantong UniversityNantongPeople’s Republic of China

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