Clinical and Translational Oncology

, Volume 17, Issue 1, pp 65–73

Expression of vimentin and survivin in clear cell renal cell carcinoma and correlation with p53

  • Z.-G. Shi
  • S.-Q. Li
  • Z.-J. Li
  • X.-J. Zhu
  • P. Xu
  • G. Liu
Research Article

Abstract

Purpose

This study focuses on investigating the expression correlation of vimentin, survivin and p53 in clear cell renal cell carcinoma (ccRCC) and the clinical significance.

Methods

The mRNA and protein expression levels of the vimentin, survivin and p53 were determined in ccRCC and adjacent normal renal tissues, using quantitative real-time-polymerase chain reaction (qRT-PCR) and Western blot. We detected the expression and localization of vimentin, survivin and p53 protein in ccRCC by immunohistochemistrical SP method and analyzed the relationships among clinical pathologic parameters and patient prognosis.

Results

The expression of vimentin and survivin was significantly increased in ccRCC compared with adjacent normal renal tissues, which were positively correlated with the pathological grade and clinical stage (P < 0.05). p53 was highly expressed in ccRCC compared with normal tissues (P < 0.05), which was not positively correlated with the pathological grade and clinical stage (P > 0.05). Furthermore, univariate and multivariate analysis showed that high expression levels of vimentin and survivin were independent prognostic indicators for ccRCC. The levels of vimentin and survivin were positively correlated in ccRCC (r = 0.428, P < 0.01).

Conclusions

Reliable basis about biological behavior and prognosis judgments of ccRCC can be provided by combining detection of vimentin and survivin. Foundation and new ideas for gene therapy of ccRCC may be provided by further studying the relationship among vimentin, survivin and p53 in ccRCC.

Keywords

Vimentin Survivin ccRCC p53 Relationship 

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Copyright information

© Federación de Sociedades Españolas de Oncología (FESEO) 2014

Authors and Affiliations

  • Z.-G. Shi
    • 1
  • S.-Q. Li
    • 2
  • Z.-J. Li
    • 1
  • X.-J. Zhu
    • 1
  • P. Xu
    • 1
  • G. Liu
    • 1
  1. 1.Department of UrologyThe First Affiliated Hospital of Henan University of Science and TechnologyLuoyangPeople’s Republic of China
  2. 2.Molecular Medicine Key Laboratory of Liver Injury and Repair, Medical CollegeHenan University of Science and TechnologyLuoyangPeople’s Republic of China

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