Clinical and Translational Oncology

, Volume 15, Issue 5, pp 358–363 | Cite as

Polymorphisms in HIF-1alpha affect presence of lymph node metastasis and can influence tumor size in squamous-cell carcinoma of the glottic larynx

  • F. Mera-Menéndez
  • A. Hinojar-GutiérrezEmail author
  • M. Guijarro Rojas
  • J. García de Gregorio
  • E. Mera-Menéndez
  • J. J. Sánchez
  • M. Quintanilla
  • L. CerezoEmail author
  • C. GamalloEmail author



HIF-1alpha plays a key role in the development and progression of cancer. Its polymorphic variants C1772T and G1790A have been associated with greater susceptibility to cancer and increased tumor progression.


We determined the distribution of these polymorphisms among 121 patients with glottic cancer and 154 healthy volunteers by PCR–RFLP. We also analyzed the relationship between the presence of these polymorphisms and various clinicopathologic variables.


Advanced tumors (T3–T4) were associated with the TT variant (p = 0.036), which was present in 75 % of T4 tumors (p = 0.008).

Among patients with nodal metastasis (N+), 41.7 and 22 % were carrying the TT and GA variants, respectively, compared with 9.4 and 2 % of the patients with no metastasis (N0), (p = 0.006 and p = 0.032).


The presence of the TT and GA variants were associated with lymph node metastasis, while the presence of the TT variant can be associated with larger tumor size.


HIF-1 HIF-1alpha Polymorphism Glottic cancer Laryngeal cancer 



We thank Thomas O’Boyle for editorial assistance and Yolanda Suárez Del Val for her help with the molecular analysis. This work was supported by the Foundation for Biomedical Research, Hospital Universitario de La Princesa.

Supplementary material

12094_2012_930_MOESM1_ESM.doc (322 kb)
Supplementary material 1 (DOC 321 kb)
12094_2012_930_MOESM2_ESM.doc (78 kb)
Supplementary material 2 (DOC 78 kb)


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Copyright information

© Federación de Sociedades Españolas de Oncología (FESEO) 2012

Authors and Affiliations

  • F. Mera-Menéndez
    • 1
  • A. Hinojar-Gutiérrez
    • 1
    Email author
  • M. Guijarro Rojas
    • 2
  • J. García de Gregorio
    • 1
  • E. Mera-Menéndez
    • 3
  • J. J. Sánchez
    • 4
  • M. Quintanilla
    • 5
  • L. Cerezo
    • 6
    Email author
  • C. Gamallo
    • 2
    • 7
    Email author
  1. 1.Departamento de Otorrinolaringología, Hospital Universitario de La PrincesaUniversidad Autónoma de MadridMadridSpain
  2. 2.Departamento de Anatomía Patológica, Hospital Universitario de La PrincesaUniversidad Autónoma de MadridMadridSpain
  3. 3.Departamento de Sistemas Informáticos y ComputaciónUniversidad Complutense de MadridMadridSpain
  4. 4.Departamento de Medicina PreventivaFacultad de Medicina, Universidad Autónoma de MadridMadridSpain
  5. 5.Instituto de investigaciones Biomédicas “Alberto Sols”, CSIC-UAMUniversidad Autónoma de MadridMadridSpain
  6. 6.Departamento de Oncología Radioterápica, Hospital Universitario de la PrincesaUniversidad Autónoma de MadridMadridSpain
  7. 7.Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERHED)BarcelonaSpain

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