Clinical and Translational Oncology

, Volume 15, Issue 1, pp 26–32 | Cite as

Efficiency and mechanism of intracellular paclitaxel delivery by novel nanopolymer-based tumor-targeted delivery system, NanoxelTM

  • Alka Madaan
  • Pratibha Singh
  • Anshumali Awasthi
  • Ritu Verma
  • Anu T. Singh
  • Manu Jaggi
  • Shiva Kant Mishra
  • Sadanand Kulkarni
  • Hrishikesh Kulkarni
Research Article



An increasing research interest has been directed toward nanoparticle-based drug delivery systems for their advantages. The appropriate amalgamation of pH sensitivity and tumor targeting is a promising strategy to fabricate drug delivery systems with high efficiency, high selectivity and low toxicity.

Materials and Methods

A novel pH sensitive Cremophor-free paclitaxel formulation, NanoxelTM, was developed in which the drug is delivered as nanomicelles using a polymeric carrier that specifically targets tumors. The efficiency and mechanism of intracellular paclitaxel delivery by NanoxelTM was compared with two other commercially available paclitaxel formulations: AbraxaneTM and IntaxelTM, using different cell lines representing target cancers [breast, ovary and non-small cell lung carcinoma (NSCLC)] by transmission electron microscopy and quantitative intracellular paclitaxel measurements by high performance liquid chromatography.


The data obtained from the present study revealed that the uptake of nanoparticle-based formulations NanoxelTM and AbraxaneTM is mediated by the process of endocytosis and the uptake of paclitaxel was remarkably superior to IntaxelTM in all cell lines tested. Moreover, the intracellular uptake of paclitaxel in NanoxelTM- and AbraxaneTM-treated groups was comparable. Hence, the nanoparticle-based formulations of paclitaxel (NanoxelTM and AbraxaneTM) are endowed with higher efficiency to deliver the drug to target cells as compared to the conventional Cremophor-based formulation.


NanoxelTM appears to be of great promise in tumor targeting and may provide an advantage for paclitaxel delivery into cancer cells.


Paclitaxel Delivery system Tumor Naoxel Nanoparticle Intaxel 


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Copyright information

© Federación de Sociedades Españolas de Oncología (FESEO) 2012

Authors and Affiliations

  • Alka Madaan
    • 1
  • Pratibha Singh
    • 1
  • Anshumali Awasthi
    • 1
  • Ritu Verma
    • 1
  • Anu T. Singh
    • 1
  • Manu Jaggi
    • 1
  • Shiva Kant Mishra
    • 3
  • Sadanand Kulkarni
    • 3
  • Hrishikesh Kulkarni
    • 2
  1. 1.Dabur Research FoundationsGhaziabadIndia
  2. 2.Fresenius Kabi Asia Pacific LimitedWanchaiHong Kong
  3. 3.Medical Affairs and Clinical ResearchFresenius Kabi India Private LimitedGurgaonIndia

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