Clinical and Translational Oncology

, Volume 13, Issue 10, pp 747–752 | Cite as

Change of telomerase activity in peripheral whole blood of head and neck squamous cell carcinoma patients before and after surgery: a pilot study

  • Yong Seop Lee
  • Kyung Tae
  • Seung Hwan Lee
  • Seok Hyun Cho
  • Jin Hyeok Jeong
  • Hyun Jung Min
  • Yong Bae Ji
Research Articles

Abstract

Background

The purpose of this study was to evaluate telomerase activity in peripheral whole blood from head and neck squamous cell carcinoma (HNSCC) patients as a biomarker for diagnosis of HNSCC or detection of recurrence during follow-up.

Materials and methods

Telomerase activity was measured from peripheral whole blood extracts by telomerase repeat amplification protocol (TRAP) in HNSCC patients before and after surgery and in a control group. Sixty-two HNSCC patients and 42 control subjects were included.

Results

Telomerase activity was found in 41 out of 62 (66.1%) HNSCC patients before surgery and in 8 out of 42 (19.0%) controls (p<0.001). Among 41 HNSCC patients who showed positive telomerase activity before surgery, 32 (78.1%) showed a conversion of telomerase activity to negative after surgery. In follow-up, 6 out of 8 (75%) showed conversion of telomerase activity from negative to positive after recurrence. Telomerase activity was changed to negative in 4 out of 6 (66%) recurred patients with positive telomerase activity after second surgery.

Conclusion

The telomerase activity in peripheral whole blood extracts of HNSCC patients might be a useful biomarker for detecting recurrence after treatment. Further study with larger sample size using a more sensitive detection method of telomerase activity is necessary to verify these results.

Keywords

Telomerase Head and neck cancer 

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Copyright information

© Feseo 2011

Authors and Affiliations

  • Yong Seop Lee
    • 1
  • Kyung Tae
    • 1
  • Seung Hwan Lee
    • 1
  • Seok Hyun Cho
    • 1
  • Jin Hyeok Jeong
    • 1
  • Hyun Jung Min
    • 1
  • Yong Bae Ji
    • 1
  1. 1.Department of Otolaryngology, Head and Neck Surgery College of MedicineHanyang UniversitySeongdong-gu SeoulSouth Korea

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