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Green Synthesis of the Flavor Esters with a Marine Candida parapsilosis Esterase Expressed in Saccharomyces cerevisiae

Abstract

The green synthesis of the flavor esters, n-propyl acetate, isobutyl acetate and isoamyl acetate had the advantages over the chemical synthesis. The esterase from Candida parapsilosis could transform n-propanol, isobutanol and isoamyl alcohol into n-propyl acetate, isobutyl acetate and isoamyl acetate, respectively. The esterase was expressed in Saccharomyces cerevisiae. At 30 °C for 1 d, the concentration of n-propyl acetate, isobutyl acetate and isoamyl acetate synthesized by the esterase expressed in Saccharomyces cerevisiae was 24.6 mg/100 mL, 8.3 mg/100 mL, 5.6 mg/100 mL, respectively. Expression of the esterase has a practical significance for flavor ester synthesis by green biochemical process.

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Acknowledgements

This work was supported by the Foundation of Hubei Provincial Department of Education (B2018040) and Research Fund for the Doctoral Program of Hubei University of Technology (No. BSQD14018).

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Correspondence to Chunjie Gong.

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Xue, D., Yao, D., You, X. et al. Green Synthesis of the Flavor Esters with a Marine Candida parapsilosis Esterase Expressed in Saccharomyces cerevisiae. Indian J Microbiol (2020). https://doi.org/10.1007/s12088-020-00856-9

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Keywords

  • Flavor ester
  • Esterase
  • Candida parapsilosis
  • Saccharomyces cerevisiae