Indian Journal of Microbiology

, Volume 59, Issue 4, pp 500–507 | Cite as

Solvent-Tolerant Acyltransferase from Bacillus sp. APB-6: Purification and Characterization

  • Deepak PandeyEmail author
  • Sanjay K. S. Patel
  • Rajendra Singh
  • Pradeep Kumar
  • Vikram Thakur
  • Duni ChandEmail author
Original Research article


Amidase from Bacillus sp. APB-6 with very good acyltransferase activity was purified to homogeneity with a purification fold of 3.68 and 53.20% enzyme yield. The purified protein's subunit molecular mass was determined approximately 42 kDa. Hyperactivity of the enzyme was observed at pH 7.5 (150 mM, potassium-phosphate buffer) and 50 °C of incubation. An enhancement in activity up to 42% was recorded with ethylenediaminetetraacetic acid and dithiothreitol. The kinetic parameter Km values for substrates: acetamide and hydroxylamine-hydrochloride were 73.0 and 153 mM, respectively. Further, the Vmax for acyltransferase activity was 1667 U/mg of protein and the Ki for acetamide was calculated as 37.0 mM. The enzyme showed tolerance to various organic solvents (10%, v/v) and worked well in the biphasic reaction medium. The acyltransferase activity in presence of solvents i.e. biphasic medium may prove highly favorable for the transformation of hydrophobic amides, which otherwise is not possible in simple aqueous phase.


Bacillus sp. APB-6 Amidase Acyltransferase Biphasic system Hydrophobic amides 



Department of Biotechnology, Himachal Pradesh University, Shimla is duly acknowledged for extending their research laboratory facility.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

12088_2019_836_MOESM1_ESM.doc (570 kb)
Supplementary material 1 (DOC 570 kb)


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Copyright information

© Association of Microbiologists of India 2019

Authors and Affiliations

  1. 1.Department of Reproductive BiologyAll India Institute of Medical SciencesNew DelhiIndia
  2. 2.Department of BiotechnologyHimachal Pradesh UniversityShimlaIndia
  3. 3.Faculty of Applied Sciences and BiotechnologyShoolini UniversitySolanIndia

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