Indian Journal of Microbiology

, Volume 59, Issue 3, pp 351–355 | Cite as

Genome Sequencing Revealed the Biotechnological Potential of an Obligate Thermophile Geobacillus thermoleovorans Strain RL Isolated from Hot Water Spring

  • Durgesh Narain Singh
  • Utkarsh Sood
  • Amit Kumar Singh
  • Vipin Gupta
  • Mallikarjun Shakarad
  • Charu Dogra RawatEmail author
  • Rup LalEmail author
Original research article


In the present study, we report the draft genome sequence of an obligate thermophile Geobacillus thermoleovorans strain RL isolated from Manikaran hot water spring located atop the Himalayan ranges, India. Strain RL grew optimally at 70 °C but not below 45 °C. The draft genome (3.39 Mb) obtained by Illumina sequencing contains 138 contigs with an average G + C content of 52.30%. RAST annotation showed that amino acid metabolism pathways were most dominant followed by carbohydrate metabolism. Genome-wide analysis using NCBI’s Prokaryotic Genome Annotation Pipeline revealed that strain RL encodes for a cocktail of industrially important hydrolytic enzymes glycoside hydrolase, α-and β-glucosidase, xylanase, amylase, neopullulanase, pullulanase and lipases required for white biotechnology. In addition, the presence of genes encoding green biocatalyst multicopper polyphenol oxidase (laccase) and an anticancer enzyme l-glutaminase reflects the significance of strain RL in gray and red biotechnology, respectively. Strain RL is a thermophilic multi-enzyme encoding bacterium which could be the source for the recombinant production of biotechnologically significant enzymes. In, addition whole cells of strain RL may be used in bioremediation studies.


Thermophile Geobacillus thermoleovorans Draft genome Hydrolytic enzymes Bioremediation Biotechnology 



This work was supported by a Grant to AKS (SERB N-PDF (PDF/2016/002780). DNS acknowledge Council of Scientific and Industrial Research (CSIR) for providing financial support under CSIR-Senior Research Associate (Pool Scientist’s Scheme). US and VG acknowledge University Grant Commission (UGC) and CSIR, respectively for providing Senior Research Fellowship. CDR is grateful to Dr. Manoj Khanna, Principal, Ramjas College, University of Delhi for his constant support.

Compliance with Ethical Standards

Conflict of interest

All authors declare no conflict of interest.


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Copyright information

© Association of Microbiologists of India 2019

Authors and Affiliations

  • Durgesh Narain Singh
    • 1
  • Utkarsh Sood
    • 1
    • 2
  • Amit Kumar Singh
    • 3
  • Vipin Gupta
    • 1
    • 2
  • Mallikarjun Shakarad
    • 1
  • Charu Dogra Rawat
    • 4
    Email author
  • Rup Lal
    • 1
    • 2
    Email author
  1. 1.Department of ZoologyUniversity of DelhiDelhiIndia
  2. 2.PhiXGen Private LimitedGurugramIndia
  3. 3.Department of BiotechnologyJamia Millia IslamiaNew DelhiIndia
  4. 4.Department of Zoology, Ramjas CollegeUniversity of DelhiDelhiIndia

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