Abstract
Viral hemorrhagic septicemia virus (VHSV), causing severe diseases in farmed fish, is detected and genotyped using conventional reverse-transcription PCR (cRT-PCR) targeting the nucleoprotein gene with corresponding VN F (forward) and VN R (reverse) primers. However, these primers have low sensitivity to VHSV subtype IVa; I investigated the cause for the poor cRT-PCR performance using various primer combinations. The results demonstrated that a 3′-end mismatch in the VN F primer reduced sensitivity and plays a critical role in VHSV detection by cRT-PCR.
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References
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Acknowledgements
I thank Niels Jørgen Olesen and Argelia Cuenca for technical and scientific support.
Funding
This study was funded by the National Fishery Products Quality Management Service, Republic of Korea.
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Kim, H.J. Importance of the 3′-Terminal Nucleotide of the Forward Primer for Nucleoprotein Gene Detection of Viral Hemorrhagic Septicemia Virus by Conventional Reverse-Transcription PCR. Indian J Microbiol 59, 234–236 (2019). https://doi.org/10.1007/s12088-019-00791-4
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DOI: https://doi.org/10.1007/s12088-019-00791-4