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Indian Journal of Microbiology

, Volume 59, Issue 2, pp 171–179 | Cite as

Anti-biofilm and Antibacterial Activity of Allium sativum Against Drug Resistant Shiga-Toxin Producing Escherichia coli (STEC) Isolates from Patient Samples and Food Sources

  • Sushma Bagde Bhatwalkar
  • Surendra Singh Gound
  • Rajesh Mondal
  • Rupesh K. Srivastava
  • Rajaneesh AnupamEmail author
Original Research Article
  • 59 Downloads

Abstract

Escherichia coli (E. coli) colonizes human intestinal tract and is usually harmless to the host. However, several strains of E. coli have acquired virulent genes and could cause enteric diseases, urinary tract and even brain infections. Shiga toxin producing Escherichia coli (STEC) is an enterohaemorrhagic E. coli (EHEC) which can result in bloody diarrhoea and could potentially lead to deadly heamolytic uremic syndrome (HUS). STEC is one of the important food borne pathogens that causes food poisoning leading to diarrhoea and number of STEC outbreaks have occurred across the world. The use of standard antibiotics to treat STEC infection is not recommended as it increases the production of shiga toxin which could lead to HUS. Therefore, use of alternative approaches which include use of plant products to treat STEC infections have been gaining attention. The objective of this study was to evaluate the antibacterial and anti-biofilm activity of garlic (Allium sativum) against STEC strains isolated from various patient and food samples using in vitro assays. The microbiological isolation of STEC from various patient and food samples resulted in eight STEC isolates of which seven strains were multidrug resistant. Antibacterial assay results indicated that all the strains exhibited dose dependent sensitivity towards garlic with zone of inhibition diameters ranging from 7 to 24 mm with 15 µl of fresh garlic extract (FGE). Minimum inhibitory  concentration (MIC) of FGE for isolates ranged from 30 to 140 µl/ml. Interestingly, the biofilm formation of all isolates in presence of 4% of FGE decreased by 35 to 59%. FTIR analysis indicated that treatment with 1% FGE results in compositional and content changes in the biofilm. In addition, the total carbohydrate content of biofilm was reduced by 40% upon 1% FGE treatment. The results of the present study report for the first time the antibacterial and anti-biofilm activity of garlic against STEC. The findings will enable development of novel garlic organosulfide based drugs for the prevention and treatment of STEC infections.

Keywords

Shiga-toxin producing E. coli (STEC) Garlic Antibacterial activity Disc diffusion assay Anti-biofilm 

Abbreviation

STEC

Shiga toxin producing E. coli

MIC

Minimum inhibitory concentration

FGE

Fresh garlic extract

EMB

Eosin methylene blue agar

SMAC

Sorbitol MacConkey agar

CT-SMAC

Cefixime tellurite sorbital MacConkey agar

QS

Quorum sensing

Notes

Acknowledgements

This work was financially supported by UGC-BSR research startup Grant [F, 30-12/2014 (BSR)], Govt. of India, sanctioned to RA. We acknowledge the Department of Chemistry, Dr. Harisingh Gour Central University, Sagar (MP)-India for providing FTIR instrument. We also would like to thank Dr. Kalpataru Das, Department of Chemistry, Dr. Harisingh Gour Central University, Sagar (MP)-India for providing invaluable help with FTIR data. We extend our thanks to Dr. Ashwani Kumar, Department of Botany, Dr. Harisingh Gour Central University, Sagar (MP)-India for his invaluable help with statistical analysis. SBB thanks UGC-RGNF.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and Animal Rights

No human or animal were used in the study.

Supplementary material

12088_2019_784_MOESM1_ESM.docx (60 kb)
Supplementary material 1 (DOCX 60 kb)

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Copyright information

© Association of Microbiologists of India 2019

Authors and Affiliations

  1. 1.Department of Biotechnology, School of Biological SciencesDr. Harisingh Gour Central UniversitySagarIndia
  2. 2.Department of Microbiology, School of Biological SciencesDr. Harisingh Gour Central UniversitySagarIndia
  3. 3.Department of Zoology, School of Biological SciencesDr. Harisingh Gour Central UniversitySagarIndia
  4. 4.Bacteriology DivisionNational Institute for Research in TuberculosisChennaiIndia
  5. 5.Department of BiotechnologyAll India Institute of Medical SciencesNew DelhiIndia

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