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Inhibition of Staphylococcus aureus and Pseudomonas aeruginosa Biofilm and Virulence by Active Fraction of Syzygium cumini (L.) Skeels Leaf Extract: In-Vitro and In Silico Studies

  • Kuldeep Gupta
  • Salam Pradeep Singh
  • Ajay Kumar Manhar
  • Devabrata Saikia
  • Nima D. Namsa
  • Bolin Kumar Konwar
  • Manabendra Mandal
Original Research Article
  • 30 Downloads

Abstract

Syzygium cumini L. Skeels (Myretacae family) is a native plant of the Indian subcontinent which has wide socio-economical importance and is well known for its ant diabetic activity. The present study aimed to investigate the antibiofilm activity of purified fraction (EA) from S. cumini leaf extract against P. aeruginosa and S. aureus. The EA did not show any effect on growth of P. aeruginosa and S. aureus at the concentration of 900 µg/ml. At this concentration EA showed biofilm inhibition up to 86 ± 1.19% (***P < 0.0001) and 86.40 ± 1.19% (***P < 0.0001) in P. aeruginosa and S. aureus respectively. SEM examination also confirmed the reduction in biofilm formation. Further EA also disrupted some virulence phenotypes in P. aeruginosa and S. aureus. Bioactive compounds detected by GC–MS showed their possible molecular interaction with RhlG/NADP active-site complex (PDB ID: 2B4Q), LasR-TP4 complex (PDB ID: 3JPU) and Pseudaminidase (PDB ID: 2W38) from P. aeruginosa. The in vitro biofilm inhibition, virulence factor inhibition and the mode of interaction of bioactive components in Syzygium cumini with QS proteins of bacteria reported in this study might be an affordable and effective alternative method of controlling quorum sensing/biofilm-associated infections.

Keywords

Anti-biofilm Syzygium cumini Pseudomonas aeruginosa Pyocyanin Swarming motility Swimming motility 

Abbreviations

EA

Ethyl acetate active fraction from crude extract

QS

Quorum sensing

PBS

Phosphate buffer saline

OD

Optical density

P. aeruginosa

Pseudomonas aeruginosa

S. aureus

Staphylococcus aureus

Notes

Acknowledgements

The author K. Gupta acknowledges to Department of Biotechnology, Government of India for financial support (DBT-JRF, Ref. No. DBT-JRF/2011-12/315 dated 19/10/2011). The authors also express their gratitude to Botanical Survey of India (BSI), Shillong, India, to help in the identification of plant sample and SAIC Tezpur University for SEM images.

Supplementary material

12088_2018_770_MOESM1_ESM.doc (32 kb)
Supplementary material 1 (DOC 31 kb)
12088_2018_770_MOESM2_ESM.pptx (9.6 mb)
Supplementary material 2 (PPTX 9867 kb)
12088_2018_770_MOESM3_ESM.docx (14 kb)
Supplementary material 3 (DOCX 14 kb)

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Copyright information

© Association of Microbiologists of India 2018

Authors and Affiliations

  • Kuldeep Gupta
    • 1
  • Salam Pradeep Singh
    • 1
  • Ajay Kumar Manhar
    • 1
  • Devabrata Saikia
    • 1
  • Nima D. Namsa
    • 1
  • Bolin Kumar Konwar
    • 1
  • Manabendra Mandal
    • 1
  1. 1.Department of Molecular Biology and BiotechnologyTezpur University (A Central University)Napaam, TezpurIndia

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