Indian Journal of Microbiology

, Volume 59, Issue 1, pp 51–57 | Cite as

Contraceptive Sperm Agglutinating Proteins Identified in Staphylococcus warneri, Natural Microflora of an Infertile Woman

  • Neeraj Chandra Pant
  • Ravinder Singh
  • Aditi Chauhan
  • Vijaya Gupta
  • Ravimohan S. Mavuduru
  • Vijay Prabha
  • Prince SharmaEmail author
Original Research Article


Staphylococcus warneri, isolated from the cervix of an adult female with unexplained infertility, was found to agglutinate human spermatozoa in vitro leading to their death. A genomic library of S. warneri was generated using pSMART-Escherichia coli vector-host system. Approximately 3500 transformants were screened and four showed sperm agglutinating activity. Sperm agglutinating proteins (SAPs) were partially purified from the positive transformants and were found to agglutinate sperms in vitro. Cloned ORFs in positive transformants were sequenced and ORF finder identified them as endonuclease, accessory secretory protein-Asp1, accessory secretory protein-Asp2 and signal transduction protein. Mannose was found to competitively inhibit sperm agglutination, indicating that SAPs in S. warneri bind to mannose in glycoprotein receptors on the surface of sperms for agglutination. This is the first report on identification of SAPs which may be responsible for unexplained infertility in women and may be used as contraceptive agents.


Staphylococcus warneri Spermicide SAPs Sperm agglutination Contraceptive 

Supplementary material

12088_2018_766_MOESM1_ESM.docx (121 kb)
Supplementary material 1 (DOCX 120 kb)


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Copyright information

© Association of Microbiologists of India 2018

Authors and Affiliations

  • Neeraj Chandra Pant
    • 1
  • Ravinder Singh
    • 1
  • Aditi Chauhan
    • 1
  • Vijaya Gupta
    • 1
  • Ravimohan S. Mavuduru
    • 2
  • Vijay Prabha
    • 1
  • Prince Sharma
    • 1
    Email author
  1. 1.Department of Microbiology, South Campus, Basic Medical Science (Block I)Panjab UniversityChandigarhIndia
  2. 2.Department of UrologyPGIMERChandigarhIndia

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