Genome Engineering Approaches to Improve Nosokomycin A Production by Streptomyces ghanaensis B38.3
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Here we describe our efforts to improve the levels of phosphoglycolipid antibiotic nosokomycin A production by Streptomyces ghanaensis ATCC14672 via genome engineering approaches. Introduction of two extra copies of leucyl tRNA (UUA) gene bldA and one copy of moenomycin biosynthesis gene cluster led, on average, to threefold increase in nosokomycin A titers (up to 1.5 mg/L). Our results validate genome engineering approach as a viable strategy to improve moenomycin production.
KeywordsStreptomyces ghanaensis Moenomycins Genome engineering Integrative vectors
This work was supported by grants from the Ministry of Education and Science of Ukraine BG-46F and BG-41Nr (to V.F. and B.O., respectively).
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Conflict of interest
All authors declare that they have no conflict of interest.
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