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Indian Journal of Microbiology

, Volume 59, Issue 1, pp 109–111 | Cite as

Genome Engineering Approaches to Improve Nosokomycin A Production by Streptomyces ghanaensis B38.3

  • Yuriy Kuzhyk
  • Maria Lopatniuk
  • Andriy Luzhetskyy
  • Victor Fedorenko
  • Bohdan Ostash
Short Communications
  • 60 Downloads

Abstract

Here we describe our efforts to improve the levels of phosphoglycolipid antibiotic nosokomycin A production by Streptomyces ghanaensis ATCC14672 via genome engineering approaches. Introduction of two extra copies of leucyl tRNA (UUA) gene bldA and one copy of moenomycin biosynthesis gene cluster led, on average, to threefold increase in nosokomycin A titers (up to 1.5 mg/L). Our results validate genome engineering approach as a viable strategy to improve moenomycin production.

Keywords

Streptomyces ghanaensis Moenomycins Genome engineering Integrative vectors 

Notes

Acknowledgements

This work was supported by grants from the Ministry of Education and Science of Ukraine BG-46F and BG-41Nr (to V.F. and B.O., respectively).

Compliance with Ethical Standards

Conflict of interest

All authors declare that they have no conflict of interest.

Supplementary material

12088_2018_761_MOESM1_ESM.pdf (445 kb)
Supplementary material 1 (PDF 445 kb)

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Copyright information

© Association of Microbiologists of India 2018

Authors and Affiliations

  1. 1.Department of Genetics and BiotechnologyIvan Franko National University of LvivLvivUkraine
  2. 2.Actinobacteria Metabolic Engineering Group, Helmholtz-Institute for Pharmaceutical Research SaarlandSaarland UniversitySaarbrückenGermany

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