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Heavy Metals Scavenging Potential of Trichoderma asperellum and Hypocrea nigricans Isolated from Acid Soil of Jharkhand

  • Sudarshan Maurya
  • Rashk-E-Eram
  • S. K. Naik
  • J. S. Choudhary
  • S. Kumar
Original Research Article

Abstract

Trichoderma asperellum (NAIMCC-F-03167) and Hypocrea nigricans (NAIMCC-F-03168) were isolated from the acidic soil of the vicinity of Litchi orchard, Ranchi, Jharkhand and were characterized on the basis of morphological, molecular and biochemical features. Both strains are fast growing, light to dark green, highly sporulative and have ability to cover 90 mm Petri dish within 96 h of inoculation. Biochemcial estimation of both isolates indicated significant cellulase and phosphate solubilisation activity. Highest cellulase activity was observed in T. asperellum (5.63 cm) followed by H. nigricans (5.10 cm) and phosphate solubilisation index was observed maximum in T. asperellum (1.93) followed by H. nigricans (1.39). Moreover, these isolates were molecularly identified on the basis of ribosomal DNA based sequences database and phylogenetic analysis in NCBI GenBank as T. asperellum (NCBI-KM 438015) and H. nigricans (NCBI-KJ910335). Negetive effect on sporulation of Lead (Pb) and Cadmium (Cd) was observed while in heavy metal scavenging potential, T. asperellum (88.9% Cd) showed highest scavenging potential followed by H. nigricans (87.2% Cd) while in Pb scavenging potential, H. nigricans (88% Pb) followed highest scavenging potential followed by T. asperellum (81.30% Pb) after 21 days of inoculation from 30 µg/ml heavy metals concentrated broth medium. If both potential bioagents can apply in Cd and Pb affected soil/water will be helpful in scavenging of heavy metals as well as management of phosphorus deficiency and soilborne fungal diseases.

Keywords

T. asperellum H. nigricans Cellulase activity Phosphate solubilisation Heavy metals scavenging potential 

Notes

Acknowledgements

The authors are grateful to Dr. B. P. Bhatt, Director, ICAR-Research Complex for Eastern Region, Patna, India, for providing facility for doing this research is duly acknowledged. Authors are also thankful to Dr. S. B. Choudhary, NBPGR, Ranchi, Jharkhand for critical review of the manuscript.

Funding

Funding was provided by Indian Council of Agricultural Research, New Delhi, India.

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Copyright information

© Association of Microbiologists of India 2018

Authors and Affiliations

  • Sudarshan Maurya
    • 1
  • Rashk-E-Eram
    • 1
  • S. K. Naik
    • 1
  • J. S. Choudhary
    • 1
  • S. Kumar
    • 1
  1. 1.ICAR-Research Complex for Eastern Region, Research CentreRanchiIndia

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