Heavy Metals Scavenging Potential of Trichoderma asperellum and Hypocrea nigricans Isolated from Acid Soil of Jharkhand
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Trichoderma asperellum (NAIMCC-F-03167) and Hypocrea nigricans (NAIMCC-F-03168) were isolated from the acidic soil of the vicinity of Litchi orchard, Ranchi, Jharkhand and were characterized on the basis of morphological, molecular and biochemical features. Both strains are fast growing, light to dark green, highly sporulative and have ability to cover 90 mm Petri dish within 96 h of inoculation. Biochemcial estimation of both isolates indicated significant cellulase and phosphate solubilisation activity. Highest cellulase activity was observed in T. asperellum (5.63 cm) followed by H. nigricans (5.10 cm) and phosphate solubilisation index was observed maximum in T. asperellum (1.93) followed by H. nigricans (1.39). Moreover, these isolates were molecularly identified on the basis of ribosomal DNA based sequences database and phylogenetic analysis in NCBI GenBank as T. asperellum (NCBI-KM 438015) and H. nigricans (NCBI-KJ910335). Negetive effect on sporulation of Lead (Pb) and Cadmium (Cd) was observed while in heavy metal scavenging potential, T. asperellum (88.9% Cd) showed highest scavenging potential followed by H. nigricans (87.2% Cd) while in Pb scavenging potential, H. nigricans (88% Pb) followed highest scavenging potential followed by T. asperellum (81.30% Pb) after 21 days of inoculation from 30 µg/ml heavy metals concentrated broth medium. If both potential bioagents can apply in Cd and Pb affected soil/water will be helpful in scavenging of heavy metals as well as management of phosphorus deficiency and soilborne fungal diseases.
KeywordsT. asperellum H. nigricans Cellulase activity Phosphate solubilisation Heavy metals scavenging potential
The authors are grateful to Dr. B. P. Bhatt, Director, ICAR-Research Complex for Eastern Region, Patna, India, for providing facility for doing this research is duly acknowledged. Authors are also thankful to Dr. S. B. Choudhary, NBPGR, Ranchi, Jharkhand for critical review of the manuscript.
Funding was provided by Indian Council of Agricultural Research, New Delhi, India.
- 18.Pilon-Smits E (2005) Phytoremediation. Annu Rev Plant Biol 56:15–39. https://doi.org/10.1146/annurev.arplant.56.032604.144214 CrossRefPubMedGoogle Scholar
- 21.Cramer RA, Byrne PF, Brick MA, Panella L, Wickliffe E, Schwartz HF (2003) Characterization of Fusarium oxysporum isolates from common bean and sugar beet using pathogenicity assays and random-amplified polymorphic DNA markers. J Phytopathol 151:352–360. https://doi.org/10.1046/j.1439-0434.2003.00731.x/pdf CrossRefGoogle Scholar
- 23.Saitou N, Nei M (1987) The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biology Evol 4:406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454 Google Scholar
- 24.Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x CrossRefPubMedGoogle Scholar
- 33.Guilger M, Pasquoto-Stigliani T, Bilesky-Jose N, Grillo R, Abhilash PC, Fernandes FL, de Lima R (2017) Biogenic silver nanoparticles based on Trichoderma harzianum: synthesis, characterization, toxicity evaluation and biological activity. Sci Rep 7:44421. https://doi.org/10.1038/srep44421 CrossRefPubMedPubMedCentralGoogle Scholar
- 36.Bruns TD, White TJ, Taylor JW (1991) Fungal molecular systematic. Annu Rev Ecol Syst 22:525–564. https://doi.org/10.1146/annurev.es.22.110191.002521 CrossRefGoogle Scholar
- 39.Paranthaman SR, Karthikeyen B (2015) Bioremediation of heavy metal in paper mill effluents using Pseudomonas spp. Int J Microbiol 1:1–5Google Scholar
- 43.Cai C-X, Xu J, Deng N-F, Dong X-W, Tang H, Liang Y, Fan X-W, Li Y-Z (2016) A novel approach of utilization of the fungal conidia biomass to remove heavy metals from the aqueous solution through immobilization. Sci Rep 6:36546. https://doi.org/10.1038/srep36546 CrossRefPubMedPubMedCentralGoogle Scholar