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Indian Journal of Microbiology

, Volume 59, Issue 1, pp 116–120 | Cite as

In Vitro Antibacterial and Synergistic Effect of Chitosan-Phytochemical Conjugates Against Antibiotic Resistant Fish Pathogenic Bacteria

  • Grace N. A. Charway
  • Seulki Park
  • Daeung Yu
  • Jae-Young Je
  • Do-Hung Kim
  • Won-Kyo Jung
  • Young-Mog KimEmail author
Short Communication
  • 87 Downloads

Abstract

Chitosan-phytochemical conjugates exhibited significant antibacterial effect with minimum inhibitory concentration (MIC) ranging from 128 to 2048 µg/ml against antibiotic-resistant fish pathogenic bacteria such as Edwardseilla tarda, Vibrio harveyi and Photobacterium damselaewhich were isolated from Korean cultured fish. Furthermore, the MIC values of old-fashioned antibiotics such as erythromycin and oxytertacycline drastically reduced in combination with chitosan-phytochemical conjugates against the fish pathogenic bacteria. The combination of conjugates with erythromycin and oxytetracycline gave median ∑FIC results ranging from 0.281 to 0.625 and 0.312 to 0.625, respectively. This result indicates the synergistic antibacterial effects and an increased susceptibility against the antibiotics.

Keywords

Antibacterial effect Chitosan-phytochemical conjugate Fish pathogenic bacteria Synergy effect 

Notes

Acknowledgements

This research was funded by the Marine Biotechnology Program (20150220) funded by the Ministry of Oceans and Fisheries, Republic of Korea. The pathogen for this study was provided by the Gyeongsang National University Hospital Branch of National Culture Collection for pathogens (GNUH-NCCP).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Association of Microbiologists of India 2018

Authors and Affiliations

  1. 1.KOICA-PKNU International Graduate Program of Fisheries SciencePukyong National UniversityBusanKorea
  2. 2.Department of Food Science and TechnologyPukyong National UniversityBusanKorea
  3. 3.Institute of Food Industrialization, Institutes of Green Bio Science and TechnologySeoul National UniversityPyeongchangKorea
  4. 4.Department of Marine-Bio Convergence SciencePukyong National UniversityBusanKorea
  5. 5.Department of Aquatic Life MedicinePukyong National UniversityBusanKorea
  6. 6.Department of Biomedical EngineeringPukyong National UniversityBusanKorea
  7. 7.Marine-Integrated Bionics Research CenterPukyong National UniversityBusanKorea

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