In Vitro Antibacterial Activity of Phlorotannins from Edible Brown Algae, Eisenia bicyclis Against Streptomycin-Resistant Listeria monocytogenes
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Listeria monocytogenes (LM) is an important food borne pathogen responsible for listeriosis. Further, LM is an etiological agent associated with life threatening conditions like meningitis and encephalitis. Biofilm forming and drug resistant LM may potentially become difficult to treat infections and hence effective controlling measures are required to prevent LM infections. In view of this, the present study evaluated an anti-listerial potential of edible brown seaweed, Eisenia bicyclis, by disc diffusion and micro-dilution methods. The results of the present study suggested that the anti-listerial activity of various phlorotannins isolated form E. bicyclis were in the range of 16–256 µg/ml. Among the phlorotannins isolated, fucofuroeckol-A (FAA) exhibited the highest anti-listerial potential (MIC range 16–32 µg/ml) against LM strains tested. Further, in checker board synergy assays, FFA-streptomycin combination exhibited significant synergy (fractional inhibitory concentration index, ∑FIC < 0.5) against aminoglycoside resistant clinical strains of LM. The results of the present study suggested the potential use of edible seaweed E. bicyclis as a source of natural phlorotannins to control food borne pathogenic infections.
KeywordsAntibiotic resistance Anti-listerial agents Eisenia bicyclis Phlorotannins
This research was supported by Marine Biotechnology Program (20150220) funded by Ministry of Oceans and Fisheries, Republic of Korea. The pathogens for this study were provided by the Gyeongsang National University Hospital Branch of National Culture Collection for Pathogens (GNUH-NCCP).
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Conflict of interest
- 2.Somers EB, Wong AC (2004) Efficacy of two cleaning and sanitizing combination on Listeria monocytogenes biofilms formed at low temperature on a variety of materials in the presence of ready-to-eat meat residue. J Food Prot 67:2218–2229. https://doi.org/10.4315/0362-028X-67.10.2218 CrossRefPubMedGoogle Scholar
- 4.Jung HA, Jin SE, Ahn BR, Lee CM, Choi JS (2013) Anti-inflammatory activity of edible brown alga Eisenia bicyclis and its constituents fucosterol and phlorotannins in LPS-stimulated RAW264.7 macrophages. Food Chem Toxicol 59:199–206. https://doi.org/10.1016/j.fct.2013.05.061 CrossRefPubMedGoogle Scholar
- 7.Clinical and Laboratory Standards Institute (CLSI) (2012) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard-Ninth Edition. CLSI document M07–A9. CLSI, Wayne, Pennsylvania, USAGoogle Scholar
- 10.Nhimiyumukiza O, Kang SK, Kim HJ, Lee EH, Han HN, Kim Y, Kim D, Kim JH, Eom SH, Kim YM (2014) Synergistic Antibacterial activity of Ecklonia cava (Phaeophyceae: Laminariales) against Listeria monocytogenes (Bacillales: Listeriaceae). Fish Aquat Sci 18:1–6. https://doi.org/10.5657/KFAS.2015.0051 Google Scholar
- 14.Lee JH, Eom SH, Lee EH, Jung YJ, Kim HJ, Jo MR, Son KT, Lee HJ, Kim JH, Lee MS, Kim YM (2014) In vitro antibacterial and synergistic effect of phlorotannins isolated from edible brown seaweed Eisenia bicyclis against acne-related bacteria. Algae 29:47–55. https://doi.org/10.4490/algae.2014.29.1.047 CrossRefGoogle Scholar
- 17.Choi JG, Kang OK, Brice OO, Lee YS, Chae HS, Oh YC, Sohn DH, Park Y, Choi HG, Kim SG, Shin DW, Kwon DY (2010) Antibacterial activity of Ecklonia cava against methicillin-resistant Staphylococcus aureus and Salmonella spp. Foodborne Path Dis 7:435–441. https://doi.org/10.1089/fpd.2009.0434 CrossRefPubMedGoogle Scholar
- 18.Eom SH, Lee DS, Jung YJ, Park JH, Choi JI, Yim MJ, Jeon JM, Kim HW, Son KT, Je JY, Lee MS, Kim YM (2014) The mechanism of antibacterial activity of phlorofucofuroeckol-A against methicillin-resistant Staphylococcus aureus. Appl Microbiol Biotechnol 98:9795–9804. https://doi.org/10.1007/s00253-014-6041-8 CrossRefPubMedGoogle Scholar