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Indian Journal of Microbiology

, Volume 58, Issue 1, pp 105–108 | Cite as

In Vitro Antibacterial Activity of Phlorotannins from Edible Brown Algae, Eisenia bicyclis Against Streptomycin-Resistant Listeria monocytogenes

  • Hyo-Jung Kim
  • Chakradhar Dasagrandhi
  • Song-Hee Kim
  • Bo-Geum Kim
  • Sung-Hwan Eom
  • Young-Mog KimEmail author
Short Communication

Abstract

Listeria monocytogenes (LM) is an important food borne pathogen responsible for listeriosis. Further, LM is an etiological agent associated with life threatening conditions like meningitis and encephalitis. Biofilm forming and drug resistant LM may potentially become difficult to treat infections and hence effective controlling measures are required to prevent LM infections. In view of this, the present study evaluated an anti-listerial potential of edible brown seaweed, Eisenia bicyclis, by disc diffusion and micro-dilution methods. The results of the present study suggested that the anti-listerial activity of various phlorotannins isolated form E. bicyclis were in the range of 16–256 µg/ml. Among the phlorotannins isolated, fucofuroeckol-A (FAA) exhibited the highest anti-listerial potential (MIC range 16–32 µg/ml) against LM strains tested. Further, in checker board synergy assays, FFA-streptomycin combination exhibited significant synergy (fractional inhibitory concentration index, ∑FIC < 0.5) against aminoglycoside resistant clinical strains of LM. The results of the present study suggested the potential use of edible seaweed E. bicyclis as a source of natural phlorotannins to control food borne pathogenic infections.

Keywords

Antibiotic resistance Anti-listerial agents Eisenia bicyclis Phlorotannins 

Notes

Acknowledgements

This research was supported by Marine Biotechnology Program (20150220) funded by Ministry of Oceans and Fisheries, Republic of Korea. The pathogens for this study were provided by the Gyeongsang National University Hospital Branch of National Culture Collection for Pathogens (GNUH-NCCP).

Compliance with Ethical Standards

Conflict of interest

None.

Supplementary material

12088_2017_693_MOESM1_ESM.docx (96 kb)
Supplementary material 1 (DOCX 96 kb)

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Copyright information

© Association of Microbiologists of India 2017

Authors and Affiliations

  • Hyo-Jung Kim
    • 1
  • Chakradhar Dasagrandhi
    • 2
  • Song-Hee Kim
    • 1
  • Bo-Geum Kim
    • 1
  • Sung-Hwan Eom
    • 3
  • Young-Mog Kim
    • 1
    • 2
    Email author
  1. 1.Department of Food Science and TechnologyPukyong National UniversityBusanKorea
  2. 2.Marine-Integrated Bionics Research CenterPukyong National UniversityBusanKorea
  3. 3.Department of Food Science and TechnologyDongeui UniversityBusanKorea

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