Genetic Diversity of Arcobacter Species of Animal and Human Origin in Andhra Pradesh, India
- 202 Downloads
Arcobacter is an emerging foodborne pathogen having zoonotic significance. Enterobacterial repetitive intergenic consensus (ERIC) PCR and repetitive sequence-based PCR (rep-PCR) analysis of a total of 41 Arcobacter isolates revealed a greater degree of genetic diversity. ERIC-PCR genotyping distinguished 14, 13 and 12 genotypes among 16, 13 and 12 isolates of A. butzleri, A. cryaerophilus and A. skirrowii, respectively. Rep-PCR genotyping distinguished 15, 12 and 11 genotypes among 16, 13 and 12 isolates of A. butzleri, A. cryaerophilus and A. skirrowii, respectively. The discriminatory power for ERIC and rep-PCR was found to be 0.997 and 0.996, respectively. Close clustering between isolates of animal and human origin are indicative of probable zoonotic significance.
KeywordsArcobacter Discriminatory power ERIC-PCR Genetic diversity rep-PCR
Thankful to Sri Venkateswara Veterinary University, Tirupati, Andhra Pradesh, for supporting this research.
- 2.Phasipol P, Chokesajjawatee N, Luangtongkum T (2013) Comparison of repetitive sequence-based polymerase chain reaction (rep-PCR) and pulsed-field gel electrophoresis (PFGE) for genetic characterization of Arcobacter spp. Thai J Vet Med 43:307Google Scholar
- 3.Ramees TP, Rathore RS, Bagalkot PS, Sailo B, Mohan HV, Kumar A, Dhama K, Singh RK (2014) Genotyping and genetic diversity of Arcobacter butzleri and Arcobacter cryaerophilus isolated from different sources by using ERIC-PCR from India. Vet Q 34:211–217. doi: 10.1080/01652176.2014.979511 CrossRefPubMedGoogle Scholar
- 7.Collado L, Kasimir G, Perez U, Bosch A, Pinto R, Saucedo G, Huguet JM, Figueras MJ (2010) Occurrence and diversity of Arcobacter spp. along the Llobregat River catchment, at sewage effluents and in a drinking water treatment plant. Water Res 44:3696–3702. doi: 10.1016/j.watres.2010.04.002 CrossRefPubMedGoogle Scholar