Indian Journal of Microbiology

, Volume 57, Issue 2, pp 253–256 | Cite as

Development and Optimization of a Reverse Transcription Hemi-Nested PCR Primer for the Detection of Potato Mop-Top Virus at Quarantine Inspection Sites in Korea

  • S. Lee
  • J. Y. Lee
  • H. Kim
  • J. H. Kim
  • J. Y. RhoEmail author
Short Communication


Potato mop-top virus (PMTV) is classified as a plant quarantine virus in Korea. It is tested at import inspection sites. In this study, two sets of reverse transcription-hemi-nested polymerase chain reaction primers were developed to diagnose PMTV. In addition, modified- positive control plasmid was developed for the identification of false positive results in plant quarantine. The assay is expected to be useful for improving the detection and diagnosis of PMTV.


Hemi-nested polymerase chain reaction Potato mop-top virus PMTV Quarantine 



The present research was conducted by the research fund of Animal and Plant Quarantine Agency, Korea.

Supplementary material

12088_2016_623_MOESM1_ESM.docx (131 kb)
Supplementary material 1 (DOCX 131 kb)


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Copyright information

© Association of Microbiologists of India 2016

Authors and Affiliations

  1. 1.Environmental Infrastructure Research DepartmentNational Institute of Environmental ResearchIncheonKorea
  2. 2.Department of Microbiology, College of Natural SciencesDankook UniversityCheonanKorea
  3. 3.School of Earth and Environmental SciencesSeoul National UniversitySeoulKorea

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