Skip to main content

Advertisement

Log in

Simple and Rapid Method for Detecting Biofilm Forming Bacteria

  • Short Communication
  • Published:
Indian Journal of Microbiology Aims and scope Submit manuscript

Abstract

Biofilm forming bacteria play a vital role in causing infectious diseases and for enhancing the efficiency of the bioremediation process through immobilization. Different media and conditions have been reported for detecting biofilm forming bacteria, however, they are not quite rapid. Here, we propose the use of a simple medium which can be used for detecting biofilm former, and also provide a mechanism to regulate the expression of biofilm formation process.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Kalia VC (2013) Quorum sensing inhibitors: an overview. Biotechnol Adv 31:224–245. doi:10.1016/j.biotechadv.2012.10.004

    Article  CAS  PubMed  Google Scholar 

  2. Kalia VC (2014) Microbes, antimicrobials and resistance: the battle goes on. Indian J Microbiol 54:1–2. doi:10.1007/s12088-013-0443-7

    Article  CAS  PubMed  Google Scholar 

  3. Kaur G, Rajesh S, Princy SA (2015) Plausible drug targets in the Streptococcus mutans quorum sensing pathways to combat dental biofilms and associated risks. Indian J Microbiol 55:349–357. doi:10.1007/s12088-015-0534-8

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Hema M, Balasubramanian S, Princy SA (2015) Meddling Vibrio cholerae murmurs: a neoteric advancement in cholera research. Indian J Microbiol 55:121–130. doi:10.1007/s12088-015-0520-1

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Koul S, Kumar P, Kalia VC (2015) A unique genome wide approach to search novel markers for rapid identification of bacterial pathogens. J Mol Genet Med 9:194. doi:10.4172/1747-0862.1000194

    Article  Google Scholar 

  6. Koul S, Prakash J, Mishra A, Kalia VC (2015) Potential emergence of multi-quorum sensing inhibitor resistant (MQSIR) bacteria. Indian J Microbiol 56:1–18. doi:10.1007/s12088-015-0558-0

    Article  PubMed  Google Scholar 

  7. Davey ME, O’Toole GA (2000) Microbial biofilms: from ecology to molecular genetics. Microbiol Mol Biol Rev 64:847–867

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. González JE, Marketon MM (2003) Quorum sensing in nitrogen-fixing rhizobia. Microbiol Mol Biol Rev 67:574–592. doi:10.1128/mmbr.67.4.574-592.2003

    Article  PubMed  PubMed Central  Google Scholar 

  9. Hassan A, Usman J, Kaleem F, Omair M, Khalid A, Iqbal M (2011) Evaluation of different detection methods of biofilm formation in the clinical isolates. Braz J Infect Dis 15:305–311. doi:10.1016/S1413-8670(11)70197-0

    Article  PubMed  Google Scholar 

  10. Knobloch JK, Horstkotte MA, Rohde H, Mack D (2002) Evaluation of different detection methods of biofilm formation in Staphylococcus aureus. Med Microbiol Immun 191:101–106. doi:10.1007/s00430-002-0124-3

    Article  CAS  Google Scholar 

  11. Liu LY, Seo J, McCanna DJ, Subbaraman LN, Jones LW (2015) Assessment of biofilm formation of E. meningoseptica, D. acidovorans and S. maltophilia in lens cases and their growth on recovery media. Contact Lens Anterior Eye 39:117–123. doi:10.1016/j.clae.2015.09.001

    Article  PubMed  Google Scholar 

  12. Nyenje ME, Green E, Ndip RN (2013) Evaluation of the effect of different growth media and temperature on the suitability of biofilm formation by Enterobacter cloacae strains isolated from food samples in South Africa. Molecules 18:9582–9593. doi:10.3390/molecules18089582

    Article  CAS  PubMed  Google Scholar 

  13. Takemura N, Noiri Y, Ehara A, Kawahara T, Noguchi N, Ebisu S (2004) Single species biofilm-forming ability of root canal isolates on gutta-percha points. Eur J Oral Sci 112:523–529. doi:10.1111/j.1600-0722.2004.00165.x

    Article  PubMed  Google Scholar 

  14. Wang H, Dong Y, Wang G, Xu X, Zhou G (2016) Effect of growth media on gene expression levels in Salmonella Typhimurium biofilm formed on stainless steel surface. Food Control 59:546–552. doi:10.1016/j.foodcont.2015.06.026

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We are thankful to the Director of CSIR-Institute of Genomics and Integrative Biology (IGIB), and CSIR project INDEPTH (BSC0111) for providing the necessary funds, facilities and moral support. Authors are also thankful to Academy of Scientific and Innovative Research (AcSIR), New Delhi.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Vipin Chandra Kalia.

Ethics declarations

Conflict of interest

Authors declare no conflict of interests.

Electronic Supplementary Material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 23 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kalia, V.C., Prakash, J., Koul, S. et al. Simple and Rapid Method for Detecting Biofilm Forming Bacteria. Indian J Microbiol 57, 109–111 (2017). https://doi.org/10.1007/s12088-016-0616-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12088-016-0616-2

Keywords

Navigation