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Indian Journal of Microbiology

, Volume 54, Issue 3, pp 274–283 | Cite as

Gene Expressing Difference in Sclerotial Formation of Morchella conica

  • Li-Jiao Chen
  • Hong-Mei Chai
  • Wei-Min Chen
  • Xing-Qi Huang
  • Yong-Chang Zhao
Original Article

Abstract

The difference of gene expression between sclerotia-producing and non-sclerotia-producing single spore isolates from Morchella conica were preliminary analyzed by mRNA differential display reverse transcription-polymerase chain reaction (RT-PCR) technique and 67 differential gene fragments were obtained. Fifty-eight of their second PCR products were cloned and sequenced. Thirteen special differential gene fragments related to sclerotial formation were validated by semi-quantitative RT-PCR. Some gene fragments had certain homologies with lipoprotein, cyclin-dependent kinase C-3, glycerophosphoryl diester phosphodiesterase, Rho GDP-dissociation inhibitor, gamma-aminobutyrate permease, OmpA family protein, Transcript antisense to ribosomal RNA protein, sodium–calcium exchange protein and keratin-associated proteins 5, 6. In addition, the putative protein of some DNA fragments had higher similarity with hypothetical protein-coding gene in NCBI database, as well as some were only putative gene fragments. All these fragments were speculated to be the functional gene associated with sclerotial formation in morel.

Keywords

mRNA differential display Morchella conica Sclerotia Semi-quantitative RT-PCR 

Notes

Acknowledgments

This work was supported by a Grant from the China Agricultural Research Systems (CARS-24).

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Copyright information

© Association of Microbiologists of India 2014

Authors and Affiliations

  • Li-Jiao Chen
    • 1
    • 2
  • Hong-Mei Chai
    • 2
    • 3
    • 4
  • Wei-Min Chen
    • 2
    • 3
    • 4
  • Xing-Qi Huang
    • 2
    • 3
    • 4
  • Yong-Chang Zhao
    • 2
    • 3
    • 4
  1. 1.College of Life SciencesYunnan UniversityKunmingPeople’s Republic of China
  2. 2.Biotechnology and Germplasm Resources InstituteYunnan Academy of Agricultural SciencesKunmingPeople’s Republic of China
  3. 3.Yunnan Provincial Key Lab of Agricultural BiotechnologyKunmingPeople’s Republic of China
  4. 4.Key Lab of Southwestern Crop Gene Resources and Germplasm InnovationMinistry of AgricultureKunmingPeople’s Republic of China

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