Prokaryotic Expression, Identification and Bioinformatics Analysis of the Mycobacterium tuberculosis Rv3807c Gene Encoding the Putative Enzyme Committed to Decaprenylphosphoryl-d-arabinose Synthesis
Decaprenylphosphoryl-d-arabinofuranosyl (DPA), the immediate donor for the polymerized d-Araf residues of mycobacterial arabinan, is synthesized from 5-phosphoribose-1-diphosphate (PRPP) in three-step reactions. (i) PRPP is transferred to decaprenyl-phosphate (DP) to form decaprenylphosphoryl-d-5-phosphoribose (DPPR). (ii) DPPR is dephosphorylated to form decaprenylphosphoryl-d-ribose (DPR). (iii) DPR is formed to DPA by the epimerase. Mycobacterium tuberculosis Rv3806c and heteromeric Rv3790/Rv3791 have been identified as the PRPP: decaprenyl-phosphate 5-phosphoribosyltransferase and the epimerase respectively. Rv3807c, however, as the candidate of phospholipid phosphatase, catalyzing the biosynthesis of decapreny-l-phosphoryl-ribose (DPR) from decaprenylphosphoryl-β-d-5-phosphoribose by dephosphorylating, has no direct experimental evidence of its essentiality in any species of mycobacterium. In this study, Rv3807c gene was amplified from the genome of M. tuberculosis H37Rv by PCR, and was successfully expressed in Escherichia coli BL21 (DE3) via the recombinant plasmid pColdII-Rv3807c. The resulting protein with the 6× His-tag was identified by SDS-PAGE and Western blotting. The protein was predicted through bioinformatics to contain three transmembrane domains, the N-terminal peptide, and a core structure with phosphatidic acid phosphatase type2/haloperoxidase. This study provides biochemical and bioinformatics evidence for the importance of Rv3807c in mycobacteria, and further functional studies will be conducted for validating Rv3807c as a promising phospholipid phosphatase in the synthetic pathway of DPA.
KeywordsMycobacterium tuberculosis Rv3807c Decaprenylphosphoryl-d-arabinose (DPA)
Polyacrylamide gel electrophoresis
Sodium dodecyl sulfate
This study is supported by the grants from National Natural Science Foundation of China (30970647).
Conflict of interest
The authors declare that they have no conflicts of interest.
- 9.Huang H, Scherman MS, D’Haeze W, Vereecke D, Holsters M, Crick DC et al (2005) Identification and active expression of the Mycobacterium tuberculosis gene encoding 5-phospho-α-d-ribose-1-diphosphate: decaprenyl-phosphate 5-phosphoribosyltransferase, the first enzyme committed to decaprenylphosphoryl-d-arabinose synthesis. J Biol Chem 280(26):24539–24543. doi: 10.1074/jbc.M504068200 PubMedCrossRefGoogle Scholar
- 12.Mikusova K, Huang H, Yagi T, Holsters M, Vereecke D, D’Haeze W et al (2005) Decaprenylphosphoryl arabinofuranose, the donor of the d-arabinofuranosyl residues of mycobacterial arabinan, is formed via a two-step epimerization of decaprenylphosphoryl ribose. J Bacteriol 187(23):8020–8025. doi: 10.1128/JB.187.23.8020-8025.2005 PubMedCentralPubMedCrossRefGoogle Scholar
- 13.Eoh H, Brown AC, Buetow L, Hunter WN, Parish T, Kaur D et al (2007) Characterization of the Mycobacterium tuberculosis 4-diphosphocytidyl-2-C-methyl-d-erythritol synthase: potential for drug development. J Bacteriol 189(24):8922–8927. doi: 10.1128/JB.00925-07 PubMedCentralPubMedCrossRefGoogle Scholar
- 17.Dong X, Bhamidi S, Scherman M, Xin Y, McNeil MR (2006) Development of a quantitative assay for mycobacterial endogenous arabinase and ensuing studies of arabinase levels and arabinan metabolism in Mycobacterium smegmatis. Appl Environ Microbiol 72(4):2601–2605. doi: 10.1128/AEM.72.4.2601-2605.2006 PubMedCentralPubMedCrossRefGoogle Scholar