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Optimization of Nutrient Requirements and Culture Conditions for the Production of Rhamnolipid from Pseudomonas aeruginosa (MTCC 7815) using Mesua ferrea Seed Oil

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Abstract

Environmental awareness has led to a serious consideration for biological surfactants and hence non-edible vegetable oils may serve as a substitute carbon source for bio-surfactant production (rhamnolipid) which might be an alternative to complex synthetic surfactants. There are reports of rhamnolipid production from plant based oil giving higher production than that of glucose because of their hydrophobicity and high carbon content. Therefore the contribution of non-edible oil such as Mesua ferrea seed oil could serve as a good carbon source for rhamnolipid production. Moreover the use of rhamnolipid production from non-edible plant based seed oil has not been reported elsewhere. The present work focus on the optimal production of rhamnolipid by considering both micro and macro nutrients and culture conditions using response surface methodology. The study observes that micronutrients play a significant role in rhamnolipid production from Pseudomonas aeruginosa (MTCC 7815). The investigation results with the statistically optimize parameters able to produce a higher rhamnolipid production and this methodology could be used to optimize the nutrients requirements and culture conditions. The present findings would assist in bioremediation of crude oil contaminated ecosystems.

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Acknowledgments

The authors would like to thank Yasser R. Abdel-Fattah, Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute, Alexandria, Egypt for his valuable suggestions in carrying out the statistical analysis part.

Conflict of interest

The authors have declared that no competing interests exist.

Author information

Correspondence to Salam Pradeep Singh.

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Singh, S.P., Bharali, P. & Konwar, B.K. Optimization of Nutrient Requirements and Culture Conditions for the Production of Rhamnolipid from Pseudomonas aeruginosa (MTCC 7815) using Mesua ferrea Seed Oil. Indian J Microbiol 53, 467–476 (2013). https://doi.org/10.1007/s12088-013-0403-2

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Keywords

  • Rhamnolipid
  • Non-edible oil
  • Response surface methodology
  • Carbon source