Indian Journal of Microbiology

, Volume 53, Issue 2, pp 168–174 | Cite as

Screening and Evaluation of Biosurfactant-Producing Strains Isolated from Oilfield Wastewater

  • Jianghong LiuEmail author
  • Yitong Chen
  • Ruidan Xu
  • Yunpeng Jia
Original Article


The six biosurfactant-producing strains, isolated from oilfield wastewater in Daqing oilfield, were screened. The production of biosurfactant was verified by measuring the diameter of the oil spreading, measuring the surface tension value and emulsifying capacity against xylene, n-pentane, kerosene and crude oil. The experimental result showed three strains (S2, S3, S6) had the better surface activity. Among the three strains, the best results were achieved when using S2 strain. The diameter of the oil spreading of the biosurfactant produced by S2 strain was 14 cm, its critical micelle concentration (CMC) was 21.8 mg/l and the interfacial tension between crude oil and biosurfactant solution produced by S2 strain reduced to 25.7 mN/m. The biosurfactant produced by S2 strain was capable of forming stable emulsions with various hydrocarbons, such as xylene, n-pentane, kerosene and crude oil. After S2 strain treatment, the reduction rate of oil viscosity was 51 % and oil freezing point reduced by 4 °C.


Biosurfactant Emulsification activity Interfacial tensions Surface tension Critical micelle concentration (CMC) 



This work is supported by the National High-technology Research and Development Program of China (863 Program) (No.2008AA06Z304). The National High-technology Research and Development Program of China (863 Program) (No.2008AA06Z304).


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Copyright information

© Association of Microbiologists of India 2013

Authors and Affiliations

  • Jianghong Liu
    • 1
    Email author
  • Yitong Chen
    • 1
  • Ruidan Xu
    • 2
  • Yunpeng Jia
    • 1
  1. 1.Provincial Key Laboratory of Oil and Gas Chemical TechnologyCollege of Chemistry and Chemical Engineering, Northeast Petroleum UniversityDaqingChina
  2. 2.School of Life Sciences, Xiamen UniversityXiamenChina

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