Indian Journal of Microbiology

, Volume 53, Issue 2, pp 208–213 | Cite as

Chemical Constituents and Antimicrobial Activity of Indian Green Leafy Vegetable Cardiospermum halicacabum

  • R. Jeyadevi
  • T. Sivasudha
  • A. Ilavarasi
  • N. Thajuddin
Original Article


The present study was carried out to analyze chemical constituents and antibacterial activity of ethanolic leaf extract of Cardiospermum halicacabum (ECH). The FT-IR spectrum confirmed the presence of alcohols, phenols, alkanes, alkynes, aliphatic ester and flavonoids in ECH. The GC–MS analysis revealed that ECH contained about twenty four compounds. The major chemical compounds identified were cyclohexane-1, 4, 5-triol-3-one-1-carboxylic acid, benzene acetic acid, caryophyllene, phytol and neophytadiene. The ECH was screened for its antibacterial activity against different bacterial strains and anti fungal activity against Candida albicans by agar well diffusion and minimum inhibitory concentration (MIC) assay. ECH exhibited antibacterial and antifungal activity. All the tested bacterial strains showed MIC values ranging from 80 to 125 μg of extract/ml and C. albicans showed 190 μg of extract/ml as a MIC. The maximum activity ECH was observed against human pathogen Staphylococcus aureus followed by Escherichia coli and the fish pathogen Aeromonas hydrophila. ECH exhibited moderate activity against some of the tested multidrug resistant strains.


Cardiospermum halicacabum FT-IR GC–MS Antimicrobial activity Multidrug resistant strains 



We thank University Grants Commission, New Delhi, India for the financial support. A. Ilavarasi acknowledges the Department of Science and Technology (DST) for INSPIRE-JRF fellowship. We also thank Mrs. K. Kanagalakshmi and Mrs. B. Vikneswari for their assistance in interpretation of FT-IR result.


  1. 1.
    Koduru S, Grierson DS, Afolayan AJ (2006) Antimicrobial activity of Solanum aculeastrum. Pharm Biol 44(4):283–286CrossRefGoogle Scholar
  2. 2.
    Chopra RN (1980) Glossary of Indian medicinal plants. Council for Scientific and Industrial Research, New DelhiGoogle Scholar
  3. 3.
    Sadique J, Chandra T, Thenmozhi V, Elango V (1987) Biochemical modes of action of Cassia occidentalis and Cardiospermum halicacabum in inflammation. J Ethnopharmacol 19(2):201–212PubMedCrossRefGoogle Scholar
  4. 4.
    Gopalakrishnan C, Dhananjayan R, Kameswaran L (1976) Studies on the pharmacological actions of Cardiospermum halicacabum. Indian J Physiol Pharmacol 20:203–206PubMedGoogle Scholar
  5. 5.
    Asha VV, Pushpangadan P (1999) Antipyretic activity of Cardiospermum halicacabum. Indian J Exp Biol 37:411–414PubMedGoogle Scholar
  6. 6.
    Sheeba MS, Asha VV (2006) Effect of Cardiospermum halicacabum on ethanol-induced gastric ulcers in rats. J Ethnopharmacol 106:105–110PubMedCrossRefGoogle Scholar
  7. 7.
    Venkatesh Babu KC, Krishnakumari S (2006) Cardiospermum halicacabum suppresses the production of TNF-alpha and nitric oxide by human peripheral blood mononuclear cells. Afr J Biomed Res 9:95–99Google Scholar
  8. 8.
    Chisholmm J, Hopkins CY (1958) Fatty acids of the seed oil of Cardiospermum halicacabum. Can J Chem 36:1537–1540CrossRefGoogle Scholar
  9. 9.
    Rajesh Kumara G, Murugananthana K, Nandakumar B, Sahil Talwar B (2011) Isolation of anxiolytic principle from ethanolic root extract of Cardiospermum halicacabum. Phytomedicine 18:219–223CrossRefGoogle Scholar
  10. 10.
    Halimi M, Vahedi H, Lari J, Nasrabadi M (2011) Chemical composition of n-hexane extract of the fruit from Bereberise integrrima of Iran. Der Pharmacia Sinica 2:27–30Google Scholar
  11. 11.
    Jeyadevi R, Sivasudha T, Rameshkumar A, Dineshkumar L (2012) Anti-arthritic activity of the Indian leafy vegetable Cardiospermum halicacabum in Wistar rats and UPLC–QTOF–MS/MS identification of the putative active phenolic components. Inflamm Res. doi: 10.1007/s00011-012-0558-z
  12. 12.
    Nezhadali A, Parsa M (2010) Study of volatile compounds in Artemesia absinthium from Iran using HS/SPME/GC/MS. Adv Appl Sci Res 1:174–179Google Scholar
  13. 13.
    Wiegand I, Hilpert K, Hancock RE (2008) Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc 3(2):163–175PubMedCrossRefGoogle Scholar
  14. 14.
    Peng XB, Li Q, Ou LN, Jiang LF, Zeng K (2010) GC–MS, FT-IR analysis of black fungus polysaccharides and its inhibition against skin aging in mice. Int J Biol Macromol 47(2):304–307PubMedCrossRefGoogle Scholar
  15. 15.
    Havsteen B (1983) Flavonoids, a class of natural products of high pharmacological potency. Biochem Pharmacol 7:1141–1148CrossRefGoogle Scholar
  16. 16.
    Rameshkumar A, Sivasudha T, Jeyadevi R, Sangeetha B, Arul Ananth D, Smilin Bell Aseervatham G, Nagarajan N, Renganathan R, Kathiravan A (2013) In vitro antioxidant and antimicrobial activities of Merremia emarginata using thio glycolic acid-capped cadmium telluride quantum dots. Coll Surf B Biointer 101:74–82CrossRefGoogle Scholar
  17. 17.
    Senthilkumar CS, Sureshkumar M, Pandian MR (2010) In vitro antibacterial activity of crude leaf extracts from Tecoma stans (L) Juss. ex Kunth, Colues forskohlii and Pogostemon patchouli against human pathogenic bacteria. Int J Pharm Tech Res 2(1):438–442Google Scholar
  18. 18.
    Longaray Delamare AP, Moschen-Pistorello IT, Artico L, Atti-Serafini L, Echeverrigaray S (2007) Antibacterial activity of the essential oils of Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil. Food Chem 100(2):603–608CrossRefGoogle Scholar
  19. 19.
    Akond MA, Alam S, Hassan SMR, Shirin M (2009) Antibiotic resistance of Escherichia coli isolated from poultry and poultry environment of Bangladesh. Int Food Safe 11:19–23Google Scholar
  20. 20.
    Kaskhedikar M, Chhabra D (2010) Multiple drug resistance in Aeromonas hydrophila isolates of fish. Veterinary World 3(2):76–77Google Scholar
  21. 21.
    Syed R, Prasad G, Deeba F, Jamil RDK, Alshatwi AA (2011) Antibiotic drug resistance of hospital acquired Staphylococcus aureus in Andhra Pradesh—a monitoring study. AJMR 5(6):671–674Google Scholar
  22. 22.
    Iwu MW, Duncan AR, Okunji CO (1999) New antimicrobials of plant origin. In: Janick J (ed) Perspectives on new crops and new uses. ASHS Press, Alexandria, pp 457–462Google Scholar

Copyright information

© Association of Microbiologists of India 2012

Authors and Affiliations

  • R. Jeyadevi
    • 1
  • T. Sivasudha
    • 1
  • A. Ilavarasi
    • 2
  • N. Thajuddin
    • 2
  1. 1.Department of Environmental BiotechnologyBharathidasan UniversityTiruchirappalliIndia
  2. 2.Department of MicrobiologyBharathidasan UniversityTiruchirappalliIndia

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