Indian Journal of Microbiology

, Volume 53, Issue 1, pp 92–99 | Cite as

Role of Microorganisms in Emission of Nitrous Oxide and Methane in Pulse Cultivated Soil Under Laboratory Incubation Condition

  • Jyotsnarani Jena
  • Sanak Ray
  • Haragobinda Srichandan
  • Anuradha Das
  • Trupti DasEmail author
Original Article


Soil from a pulse cultivated farmers land of Odisha, India, have been subjected to incubation studies for 40 consecutive days, to establish the impact of various nitrogenous fertilizers and water filled pore space (WFPS) on green house gas emission (N2O & CH4). C2H2 inhibition technique was followed to have a comprehensive understanding about the individual contribution of nitrifiers and denitrifiers towards the emission of N2O. Nevertheless, low concentration of C2H2 (5 ml: flow rate 0.1 kg/cm2) is hypothesized to partially impede the metabolic pathways of denitrifying bacterial population, thus reducing the overall N2O emission rate. Different soil parameters of the experimental soil such as moisture, total organic carbon, ammonium content and nitrate–nitrogen contents were measured at regular intervals. Application of external N-sources under different WFPS conditions revealed the diverse role played by the indigenous soil microorganism towards green house gas emission. Isolation of heterotrophic microorganisms (Pseudomonas) from the soil samples, further supported the fact that denitrification might be prevailing during specific conditions thus contributing to N2O emission. Statistical analysis showed that WFPS was the most influential parameter affecting N2O formation in soil in absence of an inhibitor like C2H2.


Incubation Nitrification Denitrification N2CH4 



The authors are grateful to Dr. S.N. Das, Emeritus Scientist and Dr. G. Roy Chaudhury, Chief Scientist, CSIR-IMMT for their constant help and valuable suggestions while writing the manuscript. Authors are thankful to the Head, Department of Environment & Sustainability and the Director, CSIR-IMMT for their encouragement. Authors are also thankful to ISRO-GBP for funding.


  1. 1.
    Richter R, Caillol S (2011) Fighting global warming: the potential of photocatalysis against CO2, CH4, N2O, CFCs, tropospheric O3, BC and other major contributors to climate change. J Photochem Photobiol 12:1–19CrossRefGoogle Scholar
  2. 2.
    Matondo JI, Graciana P, Msibi MK (2004) Evaluation of the impact of climate change on hydrology and water resources in Swaziland: part-I. Phys Chem Earth 29:1181–1191CrossRefGoogle Scholar
  3. 3.
    Nema P, Nema RK, Rangnekar S (2010) Minimization of green house gases emission by using hybrid energy system for telephony base station site application. Renew Sustain Energy Rev 14:1635–1639CrossRefGoogle Scholar
  4. 4.
    Meinshausen M, Meinshausen N, Hare W, Raper SCB, Frieler K, Knutti R, Frame DJ, Allen MR (2009) Greenhouse-gas emission targets for limiting global warming to 2 °C see associated Correspondence: Victor. Nature 458:1158–1162PubMedCrossRefGoogle Scholar
  5. 5.
    Kreye C, Dittert K, Zheng XH, Zhang X, Lin S, Tao HB, Sat-telmacher B (2007) Fluxes of methane and nitrous oxide in water-saving rice production in north China. Nutr Cycl Agroecosyst 77:293–304CrossRefGoogle Scholar
  6. 6.
    Gerard L, Velthof GL, Kuikman PJ, Oenema O (2003) Nitrous oxide emission from animal manures applied to soil under controlled conditions. Biol Fertil Soils 37:221–230Google Scholar
  7. 7.
    Pachauri RK, Reisinger A (2007) Climate change 2007: synthesis report. Intergovernmental Panel on Climate Change, CambridgeGoogle Scholar
  8. 8.
    Canadell JG, Corinne Le Quéré CL, Raupach MR, Field CB, Buitenhuis TE, Ciais P, Conway TJ, Gillett NP, Houghton RA, Marland G (2007) Contributions to accelerating atmospheric CO2 growth from economic activity, carbon intensity, and efficiency of natural sinks. Proc Natl Acad Sci USA 104:18866–18870PubMedCrossRefGoogle Scholar
  9. 9.
    Smith P, Martino D, Cai Z, Gwary D, Janze H, Kumar P, McCarl B, Ogle S, O’Mara F, Rice C, Scholes B, Sirotenko O, Howden M, McAllister T, Pan G, Romanenkov V, Schneider U, Towprayoon S, Wattenbach M, Smith J (2008) Greenhouse gas mitigation in agriculture. Philos Trans R Soc B 363:789–813CrossRefGoogle Scholar
  10. 10.
    Uprety DC, Baruah KK, Borah L (2011) Methane in rice agriculture: a review. J Sci Ind Res 70:401–411Google Scholar
  11. 11.
    Mitra AP (2004) Uncertainty reduction in GHG inventory estimates in India: an overview. In: Mitra AP, Sharma S, Bhattacharya S, Garg A, Devotta S, Sen K (eds) Climate change and India uncertainty reduction in greenhouse gas inventory estimates. University Press, HyderabadGoogle Scholar
  12. 12.
    Kool DM, Wrage N, Zechmeister-Boltenstern S, Pfeffer M, Brus D, Oenema O, Van Groenigen JW (2010) Nitrifier denitrification can be a source of N2O from soil: a revised approach to the dual-isotope labelling method. Eur J Soil Sci 61:759–772CrossRefGoogle Scholar
  13. 13.
    Hofstra N, Boweman AF (2005) Denitrification in agricultural soils: summarizing published data and estimating global annual rates. Nutr Cycl Agroecosyst 72:267–278CrossRefGoogle Scholar
  14. 14.
    Huang S, Pant HK, Lu J (2007) Effects of water regimes on N2O emission from soils. Ecol Eng 31:9–15CrossRefGoogle Scholar
  15. 15.
    Zou J, Yanyu L, Huang Y (2009) Estimates of synthetic fertilizer N-induced direct N2O emission from Chinese croplands during 1980–2000. Environ Pollut 158:631–635PubMedCrossRefGoogle Scholar
  16. 16.
    Ramulu S, Sahoo SK, Baral SS, Das SN, Swamy YV, Chaudhury GR (2008) Estimation of nitrous oxide emission from pulse cultivation in rain fed uplands. Curr Sci 95:584–585Google Scholar
  17. 17.
    Subbarao GV, Kishii M, Nakahara K, Ishikawa T, Ban T, Tsujimoto H, George TS, Berry WL, Hash CT, Ito O (2009) Biological nitrification inhibition—is there potential for genetic interventions in the Triticeae. Breed. Sci 59:529–545CrossRefGoogle Scholar
  18. 18.
    Davidson EA (1991) Fluxes of N2O and nitric oxide from terrestrial ecosystems. In: Rogers JE, Whitman WB (eds) Microbial production and consumption of green house gases: CH4, nitrogen oxides and HaloCH4s. American Society of Microbiology, Washington, DCGoogle Scholar
  19. 19.
    Sahoo SK, Ramulu TS, Kumar M, Chaudhury GR, Das SN (2010) Emission of N2O from farmers’ field during black gram cultivation and incubation experiments in laboratory. Toxicol Environ Chem 92:1043–1052CrossRefGoogle Scholar
  20. 20.
    Zhang A, Cui L, Pan G, Li L, Hussain Q, Zhang X, Zheng J, Crowley D (2010) Effect of biochar amendment on yield and methane and nitrous oxide emissions from a rice paddy from Tai Lake plain, China. Agric Ecosyst Environ 139:469–475CrossRefGoogle Scholar
  21. 21.
    Sparks DL (1996) Methods of soil analysis. Part 3: Chemical methods, SSA book series No. 5. Soil Science Society of America/American Society of Agronomy, MadisonGoogle Scholar
  22. 22.
    American Public Health Association (1998) Standard methods for the examination of water and waste water. American Public Health Association, Washington, DCGoogle Scholar
  23. 23.
    Boemaret NE, Akhurst RJ (1988) Biochemical and physiological characterization of colony form variants in Xenorhabdus spp. (Enterobacteriaceae). J Gen Microbiol 134:751–761Google Scholar
  24. 24.
    Dobbie KE, Smith KA (2001) The effect of temperature, water-filled pore space and land use on N2O emission from an imperfectly drained gley soil. Eur J Soil Sci 52:667–673CrossRefGoogle Scholar
  25. 25.
    Davidson EA, Swank WT, Perry TO (1986) Distinguish between nitrification and denitrification as source of gaseous nitrogen production in soil. Appl Environ Microbiol 52:1280–1286PubMedGoogle Scholar
  26. 26.
    Dobbie KE, Smith KA (2003) Impact of different forms of nitrogen fertilizer on N2O emission from intensive grassland. Nutr Cycl Agroecosyst 67:37–46CrossRefGoogle Scholar
  27. 27.
    Pathak H, Gupta PK, Bhatia P, Sharma C, Karla N, Mitra AP (2004) Nitrous oxide emission from soil–plant systems. In: Mitra AP, Sharma S, Bhattacharya S, Garg A, Devotta S, Sen K (eds) Climate change and India uncertainty reduction in greenhouse gas inventory estimates. University Press, Hyderabad, pp 1–30Google Scholar
  28. 28.
    Hynes R, Knowels R (1978) Inhibition of C2H2 of ammonia oxidation in Nitrousomonas europia. FEMS Microbiol Lett 4:319–321CrossRefGoogle Scholar
  29. 29.
    Berg P, Klemedtsson L, Rosswall T (1982) Inhibitory effect of low partial pressure of C2H2 on nitrification. Soil Biochem 14:301–303CrossRefGoogle Scholar
  30. 30.
    Carrera J, Jubany I, Carvallo L, Chamy R, Lafuente J (2004) Kinetic models for nitrification inhibition by ammonium and nitrite in a suspended and an immobilized biomass system. Process Biochem 39:1159–1165CrossRefGoogle Scholar
  31. 31.
    Kool DM, Warge N, Oenema O, Dolfing J, Van Groenigen JW (2007) Oxygen exchange between (de)nitrification intermediates and H2O and its implications for source determination of NO3 and N2O: a review. Rapid Commun Mass Spectrom 21:3569–3578PubMedCrossRefGoogle Scholar
  32. 32.
    Sharon Avrahami S, Conrad R, Braker G (2002) Effect of soil ammonium concentration on N2O release and on the community structure of ammonia oxidizers and denitrifiers. Appl Environ Microbiol 68:5685–5692PubMedCrossRefGoogle Scholar
  33. 33.
    Chantigny MH, Rochette P, Angers DA, Bittman S, Buckley KE, Massé DI, Bélanger G, Eriksen-Hamel NS, Gasser MO (2010) Soil nitrous oxide emissions following band-incorporation of fertilizer nitrogen and swine manure. J Environ Qual 39:1545–1553PubMedCrossRefGoogle Scholar
  34. 34.
    Conrad R (1989) Control of CH4 production in terrestrial ecosystem. In: Andreae MO, Schimel DS (eds) Exchange of trace gases between terrestrial ecosystem and atmosphere. Wiley, ChichesterGoogle Scholar
  35. 35.
    Sass RL, Turnerand FT, Jund MF (1991) CH4 emission from rice fields as influenced by solar radiation, temperature and straw incorporation. Glob Biogeochem Cycles 5:335–350CrossRefGoogle Scholar

Copyright information

© Association of Microbiologists of India 2012

Authors and Affiliations

  • Jyotsnarani Jena
    • 1
  • Sanak Ray
    • 1
  • Haragobinda Srichandan
    • 1
  • Anuradha Das
    • 1
  • Trupti Das
    • 1
    Email author
  1. 1.CSIR-Institute of Minerals and Materials TechnologyBhubaneswarIndia

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