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Indian Journal of Microbiology

, Volume 52, Issue 4, pp 544–550 | Cite as

Assessment of Microbial Richness in Pelagic Sediment of Andaman Sea by Bacterial Tag Encoded FLX Titanium Amplicon Pyrosequencing (bTEFAP)

  • Balakrishnan Sundarakrishnan
  • Muthuirulan Pushpanathan
  • Sathyanarayanan Jayashree
  • Jeyaprakash Rajendhran
  • Natarajan Sakthivel
  • Seetharaman Jayachandran
  • Paramasamy Gunasekaran
Original Article

Abstract

Microbial diversity of 1,000 m deep pelagic sediment from off Coast of Andaman Sea was analyzed by a culture independent technique, bacterial tag encoded FLX titanium amplicon pyrosequencing. The hypervariable region of small subunit ribosomal rRNA gene covering V6–V9, was amplified from the metagenomic DNA and sequenced. We obtained 19,271 reads, of which 18,206 high quality sequences were subjected to diversity analysis. A total of 305 operational taxonomic units (OTUs) were obtained corresponding to the members of firmicutes, proteobacteria, plantomycetes, actinobacteria, chloroflexi, bacteroidetes, and verucomicrobium. Firmicutes was the predominant phylum, which was largely represented with the family bacillaceae. More than 44 % of sequence reads could not be classified up to the species level and more than 14 % of the reads could not be assigned to any genus. Thus, the data indicates the possibility for the presence of uncultivable or unidentified novel bacterial species. In addition, the community structure identified in this study significantly differs with other reports from marine sediments.

Keywords

Metagenomics 16S rRNA Pyrosequencing Microbial richness Andaman Sea 

Notes

Acknowledgments

The authors thankfully acknowledge funding from Department of Biotechnology, New Delhi (No. BT/PR-10486/BCE/08/657/2008). The authors also thank Mr. Ravi, vessel manager, NIOT for his support. Authors also acknowledge the central facilities, CAS, CEGS, NRCBS and IPLS at MKU.

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Copyright information

© Association of Microbiologists of India 2012

Authors and Affiliations

  • Balakrishnan Sundarakrishnan
    • 1
  • Muthuirulan Pushpanathan
    • 2
  • Sathyanarayanan Jayashree
    • 2
  • Jeyaprakash Rajendhran
    • 2
  • Natarajan Sakthivel
    • 1
  • Seetharaman Jayachandran
    • 1
  • Paramasamy Gunasekaran
    • 2
  1. 1.Department of Biotechnology, School of Life SciencesPondicherry UniversityPondicherryIndia
  2. 2.Department of Genetics, Centre for Excellence in Genomics Sciences, School of Biological SciencesMadurai Kamaraj UniversityMaduraiIndia

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