Characterisation of Phosphate Solubilising Bacteria in Sandy Loam Soil Under Chickpea Cropping System
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With the aim to explore the possible role of phosphate-solubilizing bacteria (PSB) in phosphorus (P) cycling in agricultural soils, we isolated PSB inhabiting naturally in the sandy loam soils under chickpea cropping of Patiala (Punjab State). A total of 31 bacterial isolates showing solubilizing activities were isolated on Pikovskaya agar plates. The potent phosphate solubilizers were selected for further characterization. These isolates were shown to belong to the genera Pseudomonas and Serratia by partial sequencing analysis of their respective 16S rDNA genes. ERIC-PCR based fingerprinting was done for tracking the survival of introduced populations of the PSB during mass inoculation of these strains under chickpea plots. The results showed positive correlation (r2 = 0.853) among soil phosphatase activity and phosphate solubilizers population, which was also positively correlated (r2 = 0.730) to available phosphorus. Identification and characterization of soil PSB for the effective plant growth-promotion broadens the spectrum of phosphate solubilizers available for field application.
KeywordsAgricultural soils Chickpea Phosphatase Phosphorus Pseudomonas Serratia
The authors acknowledge the supervision of Prof. Sunil Khanna, NIIT University, Neemrana-Rajasthan for this research work; and grant support provided by the UNDP-TIFAC, Govt. of India under the IT-SAP project.
- 3.Kang SC, Ha CG, Lee TG, Maheshwari DK (2002) Solubilization of insoluble inorganic phosphate by a soil inhabiting fungus Fomitopsis sp. PS102. Curr Sci 82:439–442Google Scholar
- 4.Pradhan N, Sukla LB (2005) Solubilization of inorganic phosphate by fungi isolated from agriculture soil. Afr J Biotechnol 5:850–854Google Scholar
- 5.Kucey RMN, Janzen HH, Legett ME (1989) Microbially mediated increase in plant available phosphorus. Adv Agron 42:198–228Google Scholar
- 6.Ponmurugan P, Gopi C (2006) In vitro production of growth regulators and phosphate activity by phosphate solubilising bacteria. Afr J Biotech 5:348–350Google Scholar
- 7.Richardson AE (2001) Prospects for using soil microorganisms to improve the acquisition of phosphorus by plants. Aust J Plant Physiol 28:897–906Google Scholar
- 10.Jackson MH (1967) Soil chemical analysis. Prentice-Hall of India, New Delhi, IndiaGoogle Scholar
- 12.Pikovskaya RI (1948) Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiology 17:362–370Google Scholar
- 13.Weisberg WA, Barns SM, Pelletier DA, Lane DJ (1991) 16S rDNA amplification for phylogenetic study. J Bacteriol 173:697–703Google Scholar
- 20.Anonymous (2005) Package of practices for crops of Punjab-Rabi. 2005–06. PAU, LudhianaGoogle Scholar