Indian Journal of Microbiology

, Volume 52, Issue 2, pp 286–288 | Cite as

Analysis of Bacterial Community in the Ginseng Soil Using Denaturing Gradient Gel Electrophoresis (DGGE)

  • Regupathy Thamizh VendanEmail author
  • Sun Hee Lee
  • Young Joon Yu
  • Young Ha Rhee
Short Communication


The objective of this work was to determine the shifts in the PCR-DGGE profiles of bacterial communities associated with the rhizosphere soil of ginseng at varying age levels. Differences in the dominance of intense DNA bands in the DGGE profile was observed over the age of the plants indicating the fluctuation in the microbial community structure. The bacterial orders of actinomycetales of Actinobacteria and Spingomonadales and Rhizobiales of α-Proteobacteria were predominant in the ginseng soil.


Korean ginseng soil Bacterial community DGGE 


  1. 1.
    Ercolini D (2004) PCR-DGGE fingerprinting: novel strategies for detection of microbes in food. J Microbiol Methods 56:297–314PubMedCrossRefGoogle Scholar
  2. 2.
    Yu WJ, Lee BJ, Nam SY, Yang DC, Yun YW (2003) Modulating effects of Korean ginseng saponins on ovarian function immature rats. Biol Pharm Bull 26:2574–2580CrossRefGoogle Scholar
  3. 3.
    Muyzer G, de Waal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59:695–700PubMedGoogle Scholar
  4. 4.
    Griffiths BS, Ritz K, Ebblewhite N, Dobson G (1999) Soil microbial community structure: effects of substrate loading rates. Soil Biol Biochem 31:145–153CrossRefGoogle Scholar
  5. 5.
    EPdeB Ferreira, Dusi AN, Xavier GR, Rumjanek NG (2008) Rhizosphere bacterial communities of potato cultivars evaluated through PCR-DGGE profiles. Pesq agropec bras Brasília 43:605–612CrossRefGoogle Scholar
  6. 6.
    Kim MS, Ahn JH, Jung MK, Yu JH, Joo DH, Kim MC, Shin HC, Kim TS, Ryu TH, Kweon SJ, Kim TS, Kim DH, Ka JO (2005) Molecular and cultivation-based characterization of bacterial community structure in rice field soil. J Microbiol Biotechnol 15:1087–1093Google Scholar

Copyright information

© Association of Microbiologists of India 2011

Authors and Affiliations

  • Regupathy Thamizh Vendan
    • 1
    Email author
  • Sun Hee Lee
    • 2
  • Young Joon Yu
    • 2
  • Young Ha Rhee
    • 2
  1. 1.Agricultural College & Research Institute, Tamil Nadu Agricultural UniversityTiruchirappalliIndia
  2. 2.Department of MicrobiologyChungnam National UniversityDaejeonKorea

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