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Indian Journal of Microbiology

, Volume 51, Issue 2, pp 217–222 | Cite as

Topical Treatment of Dermatophytic Lesion on Mice (Mus musculus) Model

  • Bindu SharmaEmail author
  • Padma Kumar
  • Suresh Chandra Joshi
Original Article

Abstract

Antidermatophytic potential of three weed plants viz. Tridax procumbens L., Capparis decidua (forsk) Edgew and Lantana camara L. were explored and experimentally induced dermatophytic lesion was topically treated in mice. Microbroth dilution method was carried out for determination of MIC and MFC of different extracts of selected plants. In animal studies, mice were experimentally inoculated with Trichophyton mentagrophytes and infected animals were topically treated with 5 mg/g terbinafine and two concentrations, i.e., 5 and 10 mg/g of test extract ointment. Complete recovery from the infection was observed on 12th day of treatment for reference drug terbinafine (5 mg/g) and 10 mg/g concentration of test extract ointment whereas 5 mg/g concentration of test extract ointment showed complete cure on 16th day of treatment. Fungal burden was also calculated by culturing skin scrapings from infected animals of different groups. Test extract ointment successfully treated induced dermatophytosis in mice without any disease recurrence incidences, thereby indicating efficacy of test extract as an excellent topical antifungal agent for the cure of dermatophytosis.

Keywords

Dermatophytosis Flavonoids Trichophyton mentagrophytes 

Notes

Acknowledgments

Authors are thankful to Head, Department of Botany and Department of Zoology, University of Rajasthan for providing all necessary facilities. Financial support of UGC, Delhi is gratefully acknowledged.

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Copyright information

© Association of Microbiologists of India 2011

Authors and Affiliations

  • Bindu Sharma
    • 1
    Email author
  • Padma Kumar
    • 1
  • Suresh Chandra Joshi
    • 2
  1. 1.Laboratory of Plant Tissue Culture and Secondary Metabolites, Department of BotanyUniversity of RajasthanJaipurIndia
  2. 2.Laboratory of Reproductive Toxicology, Department of ZoologyUniversity of RajasthanJaipurIndia

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