Indian Journal of Microbiology

, Volume 50, Issue 4, pp 438–442 | Cite as

Biosorption of Pb2+ and Zn2+ by Non-Living Biomass of Spirulina sp.

  • Rajiv Kumar Aneja
  • Gunjan Chaudhary
  • Sarabjeet Singh Ahluwalia
  • Dinesh Goyal
Original Article

Abstract

Removal of heavy metals (Pb2+, Zn2+) from aqueous solution by dried biomass of Spirulina sp. was investigated. Spirulina rapidly adsorbed appreciable amount of lead and zinc from the aqueous solutions within 15 min of initial contact with the metal solution and exhibited high sequestration of lead and zinc at low equilibrium concentrations. The specific adsorption of both Pb2+ and Zn2+ increased at low concentration and decreased when biomass concentration exceeded 0.1 g l−1. The binding of lead followed Freundlich model of kinetics where as zinc supported Langmuir isotherm for adsorption with their r2 values of 0.9659 and 0.8723 respectively. The adsorption was strongly pH dependent as the maximum lead biosorption occurred at pH 4 and 10 whereas Zn2+ adsorption was at pH 8 and 10.

Keywords

Biosorption Spirulina sp. Lead Zinc 

List of symbols

Variables

Q

Metal ion adsorbed per unit mass of Spirulina (mg g−1), at equilibrium

V

Volume of solution (l)

Ci

Initial concentration of metal ions in solution (mg l−1)

Cf

Final concentration of metal ions in solution (mg l−1)

M

Mass of biomass (g)

qmax

Langmuir parameter, maximum theoretical adsorption upon complete saturation of the surface (mg g−1)

b

Langmuir constant related to the energy of adsorption desorption (l mg−1)

k

Freundlich constant related to the strength of the adsorptive bond (mg1−n/g ln)

n

Freundlich constant related to bond distribution

References

  1. 1.
    Dudka S, Miller WP (1999) Accumulation of potentially toxic elements in plant and their transfer to human food chain. J Environ Sci Heal B 34:681–708CrossRefGoogle Scholar
  2. 2.
    Volesky B (1994) Advances in biosorption of metals: selection of biomass types. FEMS Microbiol Rev 14:291–302PubMedCrossRefGoogle Scholar
  3. 3.
    Ahluwalia SS, Goyal D (2007) Microbial and plant derived biomass for removal of heavy metals from wastewater. Bioresour Technol 98:2243–2257PubMedCrossRefGoogle Scholar
  4. 4.
    Kim DW, Che DK, Wang J, Huang CP (2002) Heavy metal removal by activated sludge: influence of Nocardia amarae. Chemosphere 46:137–142PubMedCrossRefGoogle Scholar
  5. 5.
    Zouboulis AI, Loukidou MX, Matis KA (2004) Biosorption of toxic metals from aqueous solution by bacterial strains isolated from metal-polluted soils. Process Biochem 39:909–916CrossRefGoogle Scholar
  6. 6.
    Incharoensakdi A, Kitjaharn P (2002) Zinc biosorption from aqueous solution by halotolerant cyanobacterium Aphanotheca halophytica. Curr Microbiol 45:261–264PubMedCrossRefGoogle Scholar
  7. 7.
    Yan G, Viraraghavan T (2003) Heavy-metal removal from aqueous solution by fungus Mucor rouxii. Water Res 37:4486–4496PubMedCrossRefGoogle Scholar
  8. 8.
    Oswald WJ (2003) My sixty years in applied algology. J Appl Phycol 15:99–106CrossRefGoogle Scholar
  9. 9.
    Pavasant P, Apiratikul R, Sungkhum V, Suthiparinyanont P, Wattanachira S, Marhaba TF (2006) Biosorption of Cu2+, Cd2+, Pb2+, and Zn2+ using dried marine green macroalga Caulerpa lentillifera. Bioresour Technol 97:2321–2329PubMedGoogle Scholar
  10. 10.
    Polti MA, Amaroso MJ, Abate CM (2007) Chromium(IV) resistance and removal by actinomycete strains isolated from sediments. Chemosphere 67:660–667PubMedCrossRefGoogle Scholar
  11. 11.
    Xiong J, He Z, Liu D, Mahmood Q, Yang X (2008) The role of bacteria in the heavy metals removal and growth of Sedum alfredii Hance in an aqueous medium. Chemosphere 70:489–494PubMedCrossRefGoogle Scholar
  12. 12.
    Ahluwalia SS, Goyal D (2007) Microbial and plant derived biomass for removal of heavy metals from wastewater. Bioresour Technol 98:2243–2257PubMedCrossRefGoogle Scholar
  13. 13.
    Dönmez G, Aksu Z (2002) Removal of chromium(VI) from saline wastewaters by Dunaliella species. Process Biochem 38:751–762CrossRefGoogle Scholar
  14. 14.
    Davis TA, Volesky B, Mucci A (2003) A review of the biochemistry of heavy metal biosorption by brown algae. Water Res 37:4311–4330PubMedCrossRefGoogle Scholar
  15. 15.
    Chojnacka K, Chojnacki A, Górecka H (2004) Trace element removal by Spirulina sp. from copper smelter and refinery effluents. Hydrometallurgy 73:137–153CrossRefGoogle Scholar
  16. 16.
    Mehta SK, Gaur JP (2005) Use of algae for removing heavy metal ions from wastewater: progress and prospects. Crit Rev Biotechnol 25:113–152PubMedCrossRefGoogle Scholar
  17. 17.
    Kratochvil D, Volesky B (1998) Advances in the biosorption of heavy metals. Trends Biotechnol 16:291–300CrossRefGoogle Scholar
  18. 18.
    Gong R, Ding Y, Liu H, Chen Q, Liu Z (2001) Lead biosorption and desorption by intact and pretreated Spirulina maxima biomass. Chemosphere 58:125–130CrossRefGoogle Scholar
  19. 19.
    Hong C, Shan-shan P (2005) Bioremediation potential of Spirulina toxicity and biosorption studies of lead. J Zhejiang Univ Sci B 6:171–174Google Scholar
  20. 20.
    Volesky B (1992) Removal of heavy metals by biosorption. In: Ladisch MR, Bose A (eds) Harnessing biotechnology for the 21st Century. Amer Chem Soc, Washington DC, pp 462–466Google Scholar
  21. 21.
    Zhang L, Zhao L, Yu Y, Chen C (1998) Removal of Pb2+ from aqueous solution by non-living Rhizopus nigricans. Water Res 32:1437–1444CrossRefGoogle Scholar
  22. 22.
    Langmuir I (1918) The adsorption of gases in plain surface of glass, mica, and platinum. J Am Chem Soc 40:1361–1403CrossRefGoogle Scholar
  23. 23.
    Freundlich H (1907) Uber die adsorption in lousumgen. Z F Phys Chem 57:385–471Google Scholar
  24. 24.
    Solisio C, Lodi A, Soletto D, Converti A (2008) Cadmium biosorption on Spirulina platensis biomass. Bioresour Technol 99:5933–5937PubMedCrossRefGoogle Scholar
  25. 25.
    Parent L, Campbell PGC (1994) Aluminium bioavailability to the green alga Chlorella pyrenoidosa in acidified synthetic soft water. Environ Toxicol Chem 13:587–598Google Scholar
  26. 26.
    Aksu Z, Kutsal T (1990) Comparative study for biosorption characteristic of Zn ion with C. vulgaris. Environ Technol 11:979–987CrossRefGoogle Scholar
  27. 27.
    Chojnacka K, Chojnacki A, Górecka H (2005) Biosorption of Cr3+, Cd2+ and Cu2+ ions by blue-green algae Spirulina sp: kinetics, equilibrium and the mechanism of the process. Chemosphere 59:75–84PubMedCrossRefGoogle Scholar
  28. 28.
    Mehta SK, Gaur JP (2001) Removal of Ni and Cu from single and binary metal solution by free and immobilized Chlorella vulgaris. Eur J Protistol 37:261–271CrossRefGoogle Scholar
  29. 29.
    Volesky B (1990) Removal and recovery of heavy metals by biosorption. In: Volesky B (ed) Biosorption of heavy metals. CRC Press, Boca Raton, pp 7–43Google Scholar
  30. 30.
    Kojima H, Lee KY (2001) Photosynthetic microorganisms in environmental biotechnology. Springer-Verlag, Hong KongGoogle Scholar
  31. 31.
    Sandau E, Sandau P, Pulz O (1996) Heavy metal sorption by microalgae. Acta Biotechnol 16:227–235CrossRefGoogle Scholar

Copyright information

© Association of Microbiologists of India 2011

Authors and Affiliations

  • Rajiv Kumar Aneja
    • 1
  • Gunjan Chaudhary
    • 1
  • Sarabjeet Singh Ahluwalia
    • 1
  • Dinesh Goyal
    • 1
  1. 1.Department of Biotechnology and Environmental SciencesThapar UniversityPatialaIndia

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