In Vitro Antimicrobial Activity of Acacia catechu and Its Phytochemical Analysis
- 421 Downloads
Acacia catechu, commonly known as catechu, cachou and black cutch is an important medicinal plant and an economically important forest tree. The methanolic extract of this plant was found to have antimicrobial activities against six species of pathogenic and non-pathogenic microorganisms: Bacillus subtilis, Staphylococcus aureus, Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. The maximum zone of inhibition (20 mm) was found to be exhibited against S. aureus. For this organism the minimum bactericidal concentration (MBC) of the crude extract was 1,000 μg/ml. The extract was found to be equally effective against gram positive and gram negative bacteria. The antimicrobial activity of the extract was found to be decreased during purification. The chemical constituents of organic plant extracts were separated by thin layer chromatography (TLC) and the plant extracts were purified by column chromatography and were further identified by Gas chromatography–mass selection (GC–MS) analysis. The composition of A. catechu extract had shown major components of terpene i.e. camphor (76.40%) and phytol (27.56%) along with other terpenes in minor amounts which are related with their high antibacterial and antifungal properties.
KeywordsAcacia catechu Minimum inhibitory concentration Zone of inhibition In vitro antimicrobial activity TLC GC/MS
We are grateful to Prof. YK Agarwal for the corrections and valuable comments on the manuscript. We are also thankful to Central Salt and Marine Research Institute (CSMRI), Bhavnagar for carrying out GC–MS analysis.
- 6.Arunkumar S, Muthuselvam M (2009) Analysis of phytochemical constituents and antimicrobial activities of Aloe vera L. against clinical pathogens. World J Agric Sci 5(5):572–576Google Scholar
- 7.Samie A, Obi CL, Bessong PO, Namrita L (2005) Activity profiles of fourteen selected medicinal plants from Rural Venda communities in South Africa against fifteen clinical bacterial species. Afr J Biotechnol 4(12):1443–1451Google Scholar
- 13.Clinical and Laboratory Standards Institute (CLSI) (2005) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically—sixth edition: approved standard M7-A6. CLSI, WayneGoogle Scholar
- 14.Clinical and Laboratory Standards Institute (CLSI) (2008) Reference method for broth dilution antifungal susceptibility testing of yeasts: approved standard, 3rd edn. M27-A3. CLSI, WayneGoogle Scholar
- 15.Gajera HP, Patel SV, Golakiya BA (2005) Antioxidant properties of some therapeutically active medicinal plants—an overview. JMAPS 27:91–100Google Scholar