Indian Journal of Microbiology

, Volume 50, Issue 4, pp 369–374 | Cite as

In Vitro Antimicrobial Activity of Acacia catechu and Its Phytochemical Analysis

  • Bhawna Sunil NegiEmail author
  • Bharti P. Dave
Original Article


Acacia catechu, commonly known as catechu, cachou and black cutch is an important medicinal plant and an economically important forest tree. The methanolic extract of this plant was found to have antimicrobial activities against six species of pathogenic and non-pathogenic microorganisms: Bacillus subtilis, Staphylococcus aureus, Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. The maximum zone of inhibition (20 mm) was found to be exhibited against S. aureus. For this organism the minimum bactericidal concentration (MBC) of the crude extract was 1,000 μg/ml. The extract was found to be equally effective against gram positive and gram negative bacteria. The antimicrobial activity of the extract was found to be decreased during purification. The chemical constituents of organic plant extracts were separated by thin layer chromatography (TLC) and the plant extracts were purified by column chromatography and were further identified by Gas chromatography–mass selection (GC–MS) analysis. The composition of A. catechu extract had shown major components of terpene i.e. camphor (76.40%) and phytol (27.56%) along with other terpenes in minor amounts which are related with their high antibacterial and antifungal properties.


Acacia catechu Minimum inhibitory concentration Zone of inhibition In vitro antimicrobial activity TLC GC/MS 



We are grateful to Prof. YK Agarwal for the corrections and valuable comments on the manuscript. We are also thankful to Central Salt and Marine Research Institute (CSMRI), Bhavnagar for carrying out GC–MS analysis.

Supplementary material

12088_2011_61_MOESM1_ESM.doc (13.5 mb)
Supplementary material 1 (DOC 13816 kb)


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Copyright information

© Association of Microbiologists of India 2011

Authors and Affiliations

  1. 1.Department of Life ScienceBhavnagar UniversityBhavnagarIndia

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