Indian Journal of Microbiology

, Volume 50, Issue 3, pp 355–360 | Cite as

Effect of Glomus species on physiology and biochemistry of Catharanthus roseus

Scientific Correspondence

Abstract

The present study on efficacy of different Glomus species, an arbuscular mycorrhizal (AM) fungus (G. aggregatum, G. fasciculatum, G. mosseae, G. intraradices) on various growth parameters such as biomass, macro and micronutrients, chlorophyll, protein, cytokinin and alkaloid content and phosphatase activity of pink flowered Catharanthus roseus plants showed that all Glomus species except G. intraradices enhanced the chlorophyll, protein, crude alkaloid, phosphorus, sulphur, manganese and copper contents of C. roseus plants along with phosphatase activity significantly over uninoculated plants. However only G. mosseae and G. fasciculatum exhibited superior symbiotic relationship with the plant. G. mosseae was found to be the best for increasing the crude alkaloid content (8.19%) in leaf and also in increasing the quantity of important alkaloids vincristine and vinblastine.

Keywords

Catharanthus roseus Glomus species Cytokinin Alkaloids Phosphatase activity 

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References

  1. 1.
    Neuss N and Neuss MN (1990) In: The Alkaloids, Vol. 37, Brossi A, Suffness M (Eds.), Academic Press, New York pp 229Google Scholar
  2. 2.
    Kumar VM and Mahadevan A (1984) Do secondary substances inhibit mycorrhizal association. Curr Sci 53: 377–378Google Scholar
  3. 3.
    Rao YSG, Suresh CK, Suresh NS, Mallikarjunaiah RR and Bagyaraj DJ (1989) Vesicular-arbuscular mycorrhizae in medicinal plants. Indian Phytopathol 42:476–478Google Scholar
  4. 4.
    Ratti N and Janardhanan KK (1995) Vesicular-arbuscular mycorrhizal association in some alkaloid bearing plants. In: Mycorrhizae: Biofertilizers for the Future. Adholeya A, Singh S (Eds.), Proc. of the Third National Conference on Mycorrhiza, TERI, March 13–15, pp 407–409Google Scholar
  5. 5.
    Hayman DS (1980) Mycorrhiza and crop production. Nature (London) 287:487–488CrossRefGoogle Scholar
  6. 6.
    Gupta ML and Janardhanan KK (1991) Mycorrhizal association of Glomus aggregatum with palmarosa enhances growth and biomass. Plant Soil 131:261–263CrossRefGoogle Scholar
  7. 7.
    Phillips JM and Hayman DJ (1970) Improved procedures for clearing and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Tr Br Mycol Soc 56:158–61CrossRefGoogle Scholar
  8. 8.
    Jackson ML (1973) Soil Chemical Analysis. Prentice Hall, New DelhiGoogle Scholar
  9. 9.
    Bergmeyer UH (1974) Methods of Enzymatic Analysis. Academic Press, New York, pp 856–864Google Scholar
  10. 10.
    Lowery DH, Rosebrough NJ, Farr AL and Randall AJ (1951) Protein measurement with the folin-phenol Reagent. J Biol Chem 193:265–290Google Scholar
  11. 11.
    Arnon DI (1949) Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1CrossRefPubMedGoogle Scholar
  12. 12.
    Vreman JH and Corse J (1975) Recovery of cytokinins from cation exchange resins. Physiol Plant 35:333–336CrossRefGoogle Scholar
  13. 13.
    Allen MF, Moore TS, Christensen M and Stanton N (1979) Growth of vesicular arbuscular mycorrhizal and non mycorrhizal Bouteloua gracilis in a defined medium. Mycologia 71:666–669CrossRefGoogle Scholar
  14. 14.
    Uniyal GC, Bala S, Mathur AK and Kulkarni RN (2001) Symmetry C18 column: a better choice for the analysis of Indole alkaloids of Catharanthus roseus. Phytochem Anal 12:206–210CrossRefPubMedGoogle Scholar
  15. 15.
    Cochran WG and Cox GM (1963) Experimental Designs, Agric Publishing House, New DelhiGoogle Scholar
  16. 16.
    Giovannetti M and Mosseae B (1980) An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytol 84:489–500CrossRefGoogle Scholar
  17. 17.
    Gerdemann JW and Nicolson TH (1963) Spores of mycorrhizal Endogone species extracted from soils by wet sieving and decanting. Tr Br Mycol Soc 46:235–244CrossRefGoogle Scholar
  18. 18.
    Mitchell DT and Read DJ (1981) Utilization of inorganic and organic phosphatase by the mycorrhizal endophytes of Vaccinium macrocarpon and Rhododendron ponticum. Trans Br Mycol Soc 76:255–260CrossRefGoogle Scholar
  19. 19.
    Thiagarajan TR and Ahmad MH (1994) Phosphatase activity and cytokinin content in cowpeas (Vigna unguiculata) inoculated with a vesicular arbuscular mycorrhizal fungus. Biol Fertil Soils 17:51–56CrossRefGoogle Scholar
  20. 20.
    Barea JM and Azcon Aguilar C (1982) Production of plant growth regulating substances by the vesicular-arbuscular mycorrhizal fungus Glomus mosseae. Appl Environ Microbiol 43:810–813PubMedGoogle Scholar
  21. 21.
    Van Staden J and Davey JE (1979) The synthesis, transport and metabolism of endogenous cytokinins. Plant Cell Environ 2:93–106CrossRefGoogle Scholar
  22. 22.
    Miura Y, Hirata K, Kurano N, Miyamoto K and Uchida K (1988) Formation of vinblastine in multiple shoot culture of Catharanthus roseus. Planta Medica 54:18–20CrossRefPubMedGoogle Scholar
  23. 23.
    Ganapathi B and Kargi F (1990) Recent advances in indole alkaloid production by Catharanthus roseus (Periwinkle). J Exp Botany 41:259CrossRefGoogle Scholar
  24. 24.
    Gianinazzi-Pearson V and Gianinazzi S (1978) Enzymatic studies on the metabolism of vesicular-arbuscular mycorrhiza II. Soluble alkaline phosphatase specific to mycorrhizal infection in onion roots. Physiol Plant Pathol 12:45–53CrossRefGoogle Scholar
  25. 25.
    Besford RT (1978) A phosphatase as a potential indicator of the P status of the glass house cucumber (Cucumis sativus). J Sci Food Agricul 29:87–91CrossRefGoogle Scholar
  26. 26.
    Dodd JC, Burton CC, Burns RG and Jeffries P (1987) Phosphatase activity associated with the rhizosphere of plants infected with vesicular-arbuscular mycorrhizal fungi. New Phytol 7:163–172CrossRefGoogle Scholar
  27. 27.
    Capaico LCM and Callow JA (1982) The enzymes of polyphosphate metabolism in vesicular arbuscular mycorrhizae. New Phytol 91:81–91CrossRefGoogle Scholar
  28. 28.
    Gianinazzi-Pearson V and Gianinazzi S (1989) Cellular and genetic aspects of interactions between host and fungal symbionts in mycorrhizae. Genome 31:336–341Google Scholar

Copyright information

© Association of Microbiologists of India 2010

Authors and Affiliations

  1. 1.Hislop School of BiotechnologyHislop CollegeCivil LinesIndia
  2. 2.Jaipur National UniversityJagatpuraIndia
  3. 3.Central Pollution Control BoardParivesh BhawanDelhiIndia

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