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Indian Journal of Microbiology

, Volume 50, Issue 3, pp 303–308 | Cite as

Cyclodextrin glucosyltransferase production by Bacillus megaterium NCR: evaluation and optimization of culture conditions using factorial design

  • E. M. AhmedEmail author
  • Heba A. El-Refai
Original Article

Abstract

Statistically-based experimental designs were used to optimize the production of cyclodextrin glucosyltransferase (CGTase) from a local isolate of Bacillus megaterium using shack culture fermentation. Seven cultural conditions were examined for enzyme production and specific activity using Plackett-Burman factorial design. Fermentation time and K2HPO4 level were the crucial for factors improving enzyme production process. The steepest ascent design was adopted-based on the results recorded with Plackett-Burman design. Maximal enzyme estimates (activity 56.1 U/ml, and specific activity 62.7 U/mg protein) were achieved. A verification experiment was carried out to examine model validation of this optimization.

Keywords

Bacillus megaterium Cyclodextrin glucosyltransferase Optimization Factorial design 

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Copyright information

© Association of Microbiologists of India 2010

Authors and Affiliations

  1. 1.Department of Natural and Microbial ProductsNational Research CenterDokki, CairoEgypt

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