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Indian Journal of Microbiology

, Volume 49, Issue 2, pp 188–195 | Cite as

Strain improvement of Trichoderma reesei Rut C-30 for increased cellulase production

  • He Jun
  • Yu Bing
  • Zhang Keying
  • Ding Xuemei
  • Chen Daiwen
Original Article

Abstract

The strain of Trichoderma reesei Rut C-30 was subjected to mutation after treatment with N-methyl-N′-nitro-N-nitrosoguanidine (NG) for 6 h followed by UV irradiation for 15 min. Successive mutants showed enhanced cellulase production, clear hydrolysis zone and rapid growth on Avicel-containing plate. Particularly, the mutant NU-6 showed approximately two-fold increases in activity of both FPA and CMCase in shake flask culture when grown on basal medium containing peptone (1%) and wheat bran (1%). The enzyme production was further optimized using eight different media. When a mixture of lactose and yeast cream was used as cellulase inducer, the mutant NU-6 yielded the highest enzyme and cell production with a FPase activity of 6.2 U ml−1, a CMCase activity of 54.2 U ml−1, a β-glucosidase activity of 0.39 U ml−1, and a fungal biomass of 12.6 mg ml−1. It deserved noting that the mutant NU-6 also secreted large amounts of xylanases (291.3 U ml−1). These results suggested that NU-6 should be an attractive producer for both cellulose and xylanase production.

Keywords

Cellulase Trichoderma reesei NTG and ultraviolet mutation Fermentation 

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Copyright information

© Association of Microbiologists of India 2009

Authors and Affiliations

  • He Jun
    • 1
    • 2
  • Yu Bing
    • 1
    • 2
  • Zhang Keying
    • 1
    • 2
  • Ding Xuemei
    • 1
    • 2
  • Chen Daiwen
    • 1
    • 2
  1. 1.Institute of Animal NutritionSichuan Agricultural UniversityYaan, SichuanP. R. China
  2. 2.Key Laboratory of Animal Disease-Resistance NutritionMinistry of EducationYaan, SichuanChina

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