Indian Journal of Microbiology

, Volume 48, Issue 2, pp 274–278 | Cite as

Strain improvement of Aspergillus niger for the enhanced production of asperenone

Original Article
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Abstract

The enhancement of production of asperenone (Fig. 1), an inhibitor of lipoxygenase and human platelet aggregation from Aspergillus niger CFTRI 1105, was achieved by UV and nitrous acid mutagenesis. Nitrous acid mutants exhibited increased inhibitor production when compared with UV irradiated mutants. I N 41 a first-generation nitrous acid mutant produced 5.1 fold increased asperenone over parent strain. Mutant II N 31 obtained by second-generation nitrous acid treatment produced 60.3 mg asperenone/g biomass, which was 131 fold increase when compared to first generated mutant I N 41 and 670 fold increase over the parent strain. This mutant was stable for several generations on production medium.

Keywords

Asperenone Aspergillus niger Mutagenesis Nitrous acid UV irradiation 
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References

  1. 1.
    Markwell J, Frakes LG, Brott EC, Osterman J and Wager FW (1989) Aspergillus niger mutants with increased glucose oxidase production. Appl Microbiol Biotechnol 30: 166–169CrossRefGoogle Scholar
  2. 2.
    Minjares-Carranco A, Trejo-Aguilar BA, Auilar G and Viniegra-Gonzalez G (1997) Physiological comparison between pectinase-producing mutants of Aspergillus niger adopted either to solid-state fermentation or submerged fermentation. Enzyme Microb Technol 21:25–31CrossRefGoogle Scholar
  3. 3.
    Chelius MK and Wodzinski RJ (1994) Strain improvement of Aspergillus niger for phytase production. Appl Microbial Biotechnol 41:79–83CrossRefGoogle Scholar
  4. 4.
    Mala JGS, Kamini NR and Puvanakrishnan R (2001) Strain improvement of Aspergillus niger for enhanced lipase production. J Gen Appl Microbiol 47:181–186CrossRefGoogle Scholar
  5. 5.
    Fiedurek J and Gromada A (1997) Screening and mutagenesis of molds for Improvement of the simultaneous production of catalase and glucose oxidase. Enzyme Microb Technol 20:344–347CrossRefGoogle Scholar
  6. 6.
    Schewe T, Rapoport SM and Kuhn H (1986) Enzymology and physiology of Reticulocyte lipoxygenase: comparison with other lipoxygenase. Adv Enzymology 58:191–272CrossRefGoogle Scholar
  7. 7.
    Prigge ST, Boyington JC, Faig M, Doctor KS, Gaffney BJ and Amzel LM (1997) Structure and mechanism of lipoxygenases. Biochimie 79:629–636PubMedCrossRefGoogle Scholar
  8. 8.
    Cornicelli JA and Trivedi BK (1999) 15-lipoxygenase and its inhibition: a novel therapeutic target for vascular disease. Curr Pharm Res 5:11–20Google Scholar
  9. 9.
    Steinberg D (1999) At last, direct evidence that lipoxygenase play a role in atherogenesis. J Clin Invest 31:831–883Google Scholar
  10. 10.
    Rao KCS, Divakar S, Rao AGA, Karanth NG and Sattur AP (2002) (a) Lipoxygenase inhibitor from Lactobacillus casei. Biotechnol Lett 24:511–513CrossRefGoogle Scholar
  11. 11.
    Rao KCS, Divakar S, Rao AGA, Karanth NG and Sattur AP (2002) (b) A lipoxygenase inhibitor from Aspergillus niger. Appl Microbiol Biotechnol 58:539–542CrossRefGoogle Scholar
  12. 12.
    Rao KCS, Divakar S, Babu KN, Rao AGA, Karanth NG and Sattur AP (2002) (c) Nigerloxin, a novel inhibitor of aldose reductase and lipoxygenase with free radical scavenging activity from Aspergillus niger CFR-W-105. J Antibiotic 55:789–793Google Scholar
  13. 13.
    Rao KCS, Divakar S, Rao AGA. Karanth NG, Suneetha WJ, Krishnakantha TP and Sattur AP (2002) (d) Asperenone: an inhibitor of 15-lipoxygenase and human platelet aggregation from Aspergillus niger. Biotechnol Lett 24:1967–1970CrossRefGoogle Scholar
  14. 14.
    Jefferson WE (1967) (a) The isolation and characterization of asperenone, a new phenylpolyene from Aspergillus niger. Biochemistry 6:3470–3483Google Scholar
  15. 15.
    Jefferson WE (1967) (b) Steroids and other factors influencing the accumilation of asperenone and fermentation acids by Aspergillus niger in replacement cultures. Biochemistry 6:3484–3488PubMedCrossRefGoogle Scholar
  16. 16.
    Schwartz LJ and Stauffer JF (1966) Three methods of assessing the mutagenic action of ultraviolet radiation on the fungus Emericellopsis glabra. Appl Microbiol 14: 105–109PubMedGoogle Scholar
  17. 17.
    Rabache M, Neumann J and Lavollay J (1974) Phenyl poylene D’ Aspergillus niger: Structure et properties de L’asperrubrol. Phytochemistry 13: 637–642CrossRefGoogle Scholar
  18. 18.
    Ayer WA, Muir DJ and Chakravarty P (1996) Phenolic and other metabolites of Phellinus pini, a fungus pathogenic to pine. Phytochemistry 42: 1321–1324CrossRefGoogle Scholar
  19. 19.
    Cihangir N (2002) Stimulation of gibberellic acid synthesis by Aspergillus niger in submerged culture using a precursor. World J Microbiol Biotechnol 18:727–729CrossRefGoogle Scholar

Copyright information

© Association of Microbiologists of India 2008

Authors and Affiliations

  1. 1.Fermentation Technology and Bioengineering DepartmentCentral Food Technological Research InstituteMysoreIndia

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