Inactivation of Escherichia coli O157:H7 by cinnamon extracts in carrot-kinnow mandarin blends
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We investigated the antimicrobial efficacy of cinnamon extracts in laboratory prepared Kinnow-mandarin carrot blends challenged with Escerichia coli O157:H7. Freshly squeezed carrot and kinnow-mandarin juices were mixed to obtain a typical blend, inoculated with E. coli O157:H7 cultures at 102 CFU/mL with and without cinnamon extracts (0.3%) and stored at 4, 8 and 28°C for up to 10 hours. Counts on tryptic soy agar (TSA) selective medium (Mac conkey sorbitol agar) and thin agar layer (TAL) were determined at every 2 hour. The TAL method was used for recovery of sublethally injured cells. Inactivation of E. coli O157:H7 in blends containing 0.3% cinnamon extracts were observed, with killing effects being more pronounced at 28°C, similar trends were evident with blends stored under refrigeration conditions (4 and 8°C).The decrease in counts were attributed to several factors namely, pH, storage temperature and addition of cinnamon. The results of our study indicate that cinnamon extracts could be used as an effective, natural antimicrobial for assuring consumer safety at the point of preparation of carrot-kinnow mandarin blends, which is a popular, nutritional beverage consumed in India.
KeywordsUnpasteurized Carrot kinnow-mandarin blends Cinnamon extracts Incativation E. coli O157:H7
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- 1.Zaika LL (1998) Spices and herbs: their antimicrobial activity and determination. J.Food Saf 997-118Google Scholar
- 4.(CDC) Centers for Diseases Control and Prevention. Outbreak of Escherichia coli O157:H7 infections associated with drinking unpasteurized commercial apple Juice-British Columbia, California, Colorado, and Washington (1996) Morbid Mortal Wkly Rep, 45:975Google Scholar
- 5.(CDC) Centers for Diseases Control and Prevention. Outbreak of Escherichia coli O157:H7 infection and cryptosporidiosis associated with drinking unpasteurized apple cider-Connecticut and New York, October, 1996 (1997) Morbid Mortal Wkly Rep, 464–468Google Scholar
- 6.(WHO) World Health Organization. (2001). Global food safety Agenda and health. Report and draft of the secretariat, resolution: WHA 53.15, GenevaGoogle Scholar
- 7.Gupta S, Soni, NK, Kaur P & Sood DK (1992) Verocytopathic activity of Escherichia coli O157 and other ‘O’ serogroups isolated from patients of diarrhoea. Ind J Med Res 95:71–76Google Scholar
- 8.Singh BR, Kulshreshta SB & Kapoor KN (1996) An orange juice outbreak due to enterotoxigenic Escherichia coli. J Food Sci Technol 32:504–506Google Scholar
- 9.Pal A, Ghosh S, Ramamurthy T, Yamasaki S & Tsukamoto T, etal, (1999) Shiga-toxin producing Escherichia coli from healthy cattle in a semi-urban community in Calcutta, India. Ind J Med Res 110:83–85Google Scholar
- 12.Mudgil S, Agarwal D & Ganguli A (2003) Microbiological analysis of street vended fresh squeezed carrot and kinnow-mandarin juices in Patiala city, India. Internet J Food Saf, 3:1–3Google Scholar
- 19.Nelson H, (1997) The contamination of organic produce by human sources of human salmonellosis attributable to pork. In Proc. of the 3rd Int. Symp. On the Epidemiology and Control of Salmonella pathogens in animal manures. http:/eap.mcgill.ca/SFMC 1.htm (assessed: 5 Dec. 2004). Ecological Agriculture in Pork, Washington, DC. 5–7, Aug. 1999Google Scholar
- 20.Islam M, Morgan J, Doyle M & Jiang X (2004) Fate of Escherichia coli O157:H7 in manure compose amended soil on carrots and onions grown in an environmentally controlled growth chamber. J Food Prot 67(3):25–42Google Scholar