Indian Journal of Microbiology

, Volume 47, Issue 4, pp 329–335 | Cite as

Characterization of heat resistant mutant strains of Rhizobium sp. [Cajanus] for growth, survival and symbiotic properties

  • Kiran Nehra
  • Attar S. Yadav
  • Anita R. Sehrawat
  • R. K. Vashishat
Original Article


Fourteen heat resistant mutant strains were isolated from a wild-type strain (PP201, Nod+ Fix+) of Rhizobium sp. (Cajanus) by giving it a heat shock of 43°C. These mutant strains showed a greater increase in optical density (O.D.) and a higher viable cell count in both rhizospheric and non-rhizospheric soil at high temperature. Symbiotic studies showed that pigeon pea plants inoculated with a few mutant strains had ineffective nodules (Nod+ Fix) under controlled temperature (43°C) conditions, but under natural high temperature (40–45°C) conditions, the host plants infected with all the mutant strains showed higher total shoot nitrogen than the plants inoculated with the parent strain. Four mutant strains (HR-3, HR-6, HR-10 and HR-12) were found to be highly efficient for all the symbiotic parameters, and thus have the potential to be used as bioinoculants in the North-Western regions of India during the summer season.


Heat resistant mutants Growth Survival Rhizobium sp. (CajanusSymbiotic properties 


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Copyright information

© Association of Microbiologists of India 2007

Authors and Affiliations

  • Kiran Nehra
    • 1
  • Attar S. Yadav
    • 2
  • Anita R. Sehrawat
    • 2
  • R. K. Vashishat
    • 2
  1. 1.Department of BiotechnologyChaudhary Devi Lal UniversitySirsaIndia
  2. 2.Department of GeneticsCCS Haryana Agricultural UniversityHisarIndia

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