Indian Journal of Microbiology

, 47:267 | Cite as

Inhibitory activity of pine needle tannin extracts on some agriculturally resourceful microbes

  • G. SelvakumarEmail author
  • Supradip Saha
  • S. Kundu
Short Communication


Crude extracts of water and solvent extractable tannin fractions from pine needles were found to contain tannin concentrations of 10.15% and 13.15% tannic acid equivalents respectively. Thin Layer Chromatography revealed the presence of four distinct phenolic compounds, amongst which two were tannic acid like compounds. Both the extracts were found to be inhibitory to several microbes of agricultural importance. Amongst the bacterial strains studied, Azotobacter sp (VL-A2) was able to tolerate upto 1000 ppm of crude tannin concentration without any growth inhibition. While growth of Rhizobium (VL-R1) and Bacillus halodurans (MTCC 7181) was inhibited by crude tannin concentrations of 50 and 100 ppm respectively of both water and solvent extracted tannins. Among the fungal genera, Pleurotus djamor was found to tolerate up to 10000 ppm of crude tannins, while Trichoderma virescens (MTCC 6321) and T. reesii could tolerate up to 3000 ppm of both water extractable and acetone extractable crude tannins without any growth inhibition.


Pine needle Tannin Inhibitory activity Agriculture Microbial inoculants 


  1. 1.
    Bate — Smith EC & Swain T (1962) Flavonoid compounds. In: Comparative Biochemistry. (Mason HS & Florkin AM eds). Academic Press, New York, pp 755–809Google Scholar
  2. 2.
    Castels E, Penulas J & Valentine DW (2005) Effects of plant leachates from four boreal understorey species on soil N mineralization, white spruce (Picea glauca) germination and seedling growth. Annals of Botany 95:1247–1252CrossRefGoogle Scholar
  3. 3.
    Northup RR, Yu Z, Dalgren RA & Vogt KA (1995) Polyphenol control of nitrogen release from pine litter. Nature 377:227–229CrossRefGoogle Scholar
  4. 4.
    Kanvera S, Kitunen V, Kiikkilä O, Loponen J & Smolander A (2006) Response of soil C and N transformations to tannin fractions originating from Scots pine and Norway spruce needles. Soil Biol Biochem 38:1364–1374CrossRefGoogle Scholar
  5. 5.
    Broadhurst WT & Jones RB (1978) Analysis of condensed tannins using acidified vanillin. J Sci Food Agric 29: 788–794CrossRefGoogle Scholar
  6. 6.
    Schanderi SH (1970) Methods in Food Analysis. Academic Press, New York, 660pGoogle Scholar
  7. 7.
    Harbourne JB (1989) General procedures and measurements of total phenolics. In: Methods in Plant Biochemistry, Vol. 1, Academic Press, London, pp 1–28Google Scholar
  8. 8.
    Nathan P, Law EJ & Murphy DF (1978) A laboratory method for the selection of topical antimicrobial agents. Burns 4:177–178CrossRefGoogle Scholar
  9. 9.
    Nene YL & Thapliyal PN (1979) Fungicides in plant disease control. 2nd edn. Oxford and IBH Publishing Co. New Delhi, 507pGoogle Scholar
  10. 10.
    Hernes PJ, Benner R, Cowie GL, Goni M, Bergamaschi BA & Hedges JI (2001) Tannin diagenesis in mangrove leaves from a tropical estuary: a novel molecular approach. Geochim Cosmochim Acta 65:3109–3122CrossRefGoogle Scholar
  11. 11.
    Kraus TEC, Dahlgren RA & Zasoki RJ (2003) Tannins in nutrient dynamics of forest ecosystems — a review. Plant Soil 256:41–66CrossRefGoogle Scholar
  12. 12.
    Makkar HPS & Singh B (1991) Distribution of condensed tannins (proanthocyanidins) in various fibre fractions in young and mature leaves of some oak species. Anim Feed Sci Technol 32:253–260CrossRefGoogle Scholar
  13. 13.
    Fredaliana BD (2005) The potential of aqueous and acetone extracts of galls of Quercus infectoria as antibacterial agents. Indian Journal of Pharmacology 37:26–29CrossRefGoogle Scholar
  14. 14.
    Scalbert A (1991) Antimicrobial properties of tannins. Phytochemistry 30:3875–3883CrossRefGoogle Scholar
  15. 15.
    Smith AH, Erwin Z & Mackie RI (2005) Bacterial mechanisms to overcome inhibitory effects of dietary tannins. Microbial Ecology 50:197–205PubMedCrossRefGoogle Scholar
  16. 16.
    Purohit JS, Dutta JK, Nanda RK & Banerjee R (2006) Strain improvement for tannase production from co-culture of Aspergillus foetidus and Rhizopus oryzae. Bioresource Technology 97:795–801PubMedCrossRefGoogle Scholar
  17. 17.
    Bhat TK, Singh B & Sharma OP (1998) Microbial degradation of tannins — a current perspective. Biodegradation 9:643–357CrossRefGoogle Scholar
  18. 18.
    Schultz JC, Hunter MD & Appel HM (1992) Antimicrobial activity of polyphenols mediates plant herbivore interactions In: Plant Polyphenols. Synthesis, Properties, Significance. (Hemingway RW & Laks PE eds). Plenum Press, New York, pp 673–692Google Scholar
  19. 19.
    Hopper W & Mahadevan A (1991) Utilization of catechin and its metabolites by Bradyrhizobium japonicum. Applied Microbiology and Biotechnology 35:411–415CrossRefGoogle Scholar
  20. 20.
    Yague S, Terron MC, Gonzalez T, Zapico E, Bochini P, Galetti GC & Gonzalez AE (2000) Biotreatment of tannin rich beer — factory waste water with white rot basidiomycete Coriolopsis gallica monitored by pyrolyis / gas chromatography / mass spectrometry. Mass Sectrom 14:905–910Google Scholar
  21. 21.
    Bending GD & Read DJ (1996) Effects of the soluble polyphenol tannic acid on the activities of ericoid and ectomycorrhizal fungi. Soil Biol Biochem 28:1595–1602CrossRefGoogle Scholar
  22. 22.
    Mukherjee G, Banerjee R & Patra KC (2005) Microbial transformation of tannin rich substrate to gallic acid through co-culture method. Bioresource Technology 96:949–953PubMedCrossRefGoogle Scholar
  23. 23.
    Hackl E, Pfeffer M, Donat C, Bachmann G & Boltenstern SZ (2004) Composition of the microbial communities in the mineral soil under different types of natural forest. Soil Biol Biochem 37:661–671CrossRefGoogle Scholar

Copyright information

© Association of Microbiologists of India 2007

Authors and Affiliations

  1. 1.Vivekananda Parvatiya Krishi Anusandhan Sansthan (ICAR)AlmoraIndia

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